Flow rate calibration for absolute cell counting rationale and design
| Autor: | David Barnett, Clare L. Walker |
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| Předmět: |
Histology
Computer science Sample (material) Nanotechnology Leukocyte Counts Sensitivity and Specificity Biochemistry Microsphere Leukocyte Count Leukocytes Calibration Enumeration Humans Blood Cells business.industry Reproducibility of Results General Medicine Flow Cytometry Cell counting Volumetric flow rate Internal quality Medical Laboratory Technology Research Design business Computer hardware |
| Zdroj: | Scopus-Elsevier Current Protocols in Cytometry |
| Popis: | There is a need for absolute leukocyte enumeration in the clinical setting, and accurate, reliable (and affordable) technology to determine absolute leukocyte counts has been developed. Such technology includes single platform and dual platform approaches. Derivations of these counts commonly incorporate the addition of a known number of latex microsphere beads to a blood sample, although it has been suggested that the addition of beads to a sample may only be required to act as an internal quality control procedure for assessing the pipetting error. This unit provides the technical details for undertaking flow rate calibration that obviates the need to add reference beads to each sample. It is envisaged that this report will provide the basis for subsequent clinical evaluations of this novel approach. Keywords: flow cytometry; single platform; absolute counts; quality control; CD4+ T lymphocytes |
| Databáze: | OpenAIRE |
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