Generation of KCL012 research grade human embryonic stem cell line carrying a mutation in the HTT gene
| Autor: | Neli Kadeva, Dusko Ilic, Victoria Wood, Stefano Codognotto, Laureen Jacquet, Glenda Cornwell, Heema Hewitson, Emma Stephenson, Carl Hobbs |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Huntingtin Genotype Cellular differentiation Human Embryonic Stem Cells Karyotype Fertilization in Vitro Biology medicine.disease_cause Lab Resource: Stem Cell Line 03 medical and health sciences Trinucleotide Repeats medicine Huntingtin Protein Humans Allele lcsh:QH301-705.5 Cells Cultured Medicine(all) Comparative Genomic Hybridization Mutation Polymorphism Genetic Cell Differentiation Cell Biology General Medicine Cellular Reprogramming Embryo Mammalian Embryonic stem cell Cell biology 030104 developmental biology lcsh:Biology (General) Microscopy Fluorescence Cell culture Transcription Factors Developmental Biology Human embryonic stem cell line |
| Zdroj: | Jacquet, L, Hewitson, H, Wood, V, Kadeva, N, Cornwell, G, Codognotto, S, Hobbs, C, Stephenson, E & Ilic, D 2016, ' Generation of KCL012 research grade human embryonic stem cell line carrying a mutation in the HTT gene ', Stem Cell Research, vol. 16, no. 2, pp. 264-267 . https://doi.org/10.1016/j.scr.2016.01.012 Stem Cell Research Stem Cell Research, Vol 16, Iss 2, Pp 264-267 (2016) |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2016.01.012 |
| Popis: | The KCL012 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (46 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays. |
| Databáze: | OpenAIRE |
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