Identification, cloning and characterization of the tomato TCP transcription factor family

Autor: Jacqueline Busscher-Lange, Violeta Parapunova, Ruud A. de Maagd, Michiel Lammers, Rumyana Karlova, Marco Busscher, Gerco C. Angenent, Arnaud G. Bovy
Jazyk: angličtina
Rok vydání: 2014
Předmět:
0106 biological sciences
Plasma protein binding
Plant Science
dna-binding
01 natural sciences
Homology (biology)
Solanum lycopersicum
Gene Expression Regulation
Plant
Arabidopsis
Genes
Regulator
Arabidopsis thaliana
Gene Regulatory Networks
Cloning
Molecular
Promoter Regions
Genetic
fruit-development
Phylogeny
high-throughput
time
Plant Proteins
2. Zero hunger
Genetics
0303 health sciences
EPS-1
food and beverages
Multigene Family
BIOS Applied Metabolic Systems
Laboratory of Molecular Biology
leaf development
Research Article
Protein Binding
Bioinformatics
Two-hybrid screening
Molecular Sequence Data
interference
Sequence alignment
arabidopsis-thaliana
Biology
Genes
Plant
Tomato
Chromosomes
Plant
03 medical and health sciences
Two-Hybrid System Techniques
Bioinformatica
expression
Transcription factors
Laboratorium voor Moleculaire Biologie
Amino Acid Sequence
BIOS Plant Development Systems
Gene
Transcription factor
030304 developmental biology
Yeast one-hybrid
Arabidopsis Proteins
fungi
Yeast two-hybrid
biology.organism_classification
Protein Structure
Tertiary
plant-growth
Fruit
Mutation
mads-box gene
Sequence Alignment
010606 plant biology & botany
Zdroj: BMC Plant Biology 14 (2014)
BMC Plant Biology, 14
BMC Plant Biology
ISSN: 1471-2229
Popis: Background TCP proteins are plant-specific transcription factors, which are known to have a wide range of functions in different plant species such as in leaf development, flower symmetry, shoot branching, and senescence. Only a small number of TCP genes has been characterised from tomato (Solanum lycopersicum). Here we report several functional features of the members of the entire family present in the tomato genome. Results We have identified 30 Solanum lycopersicum SlTCP genes, most of which have not been described before. Phylogenetic analysis clearly distinguishes two homology classes of the SlTCP transcription factor family - class I and class II. Class II differentiates in two subclasses, the CIN-TCP subclass and the CYC/TB1 subclass, involved in leaf development and axillary shoots formation, respectively. The expression patterns of all members were determined by quantitative PCR. Several SlTCP genes, like SlTCP12, SlTCP15 and SlTCP18 are preferentially expressed in the tomato fruit, suggesting a role during fruit development or ripening. These genes are regulated by RIN (RIPENING INHIBITOR), CNR (COLORLESS NON-RIPENING) and SlAP2a (APETALA2a) proteins, which are transcription factors with key roles in ripening. With a yeast one-hybrid assay we demonstrated that RIN binds the promoter fragments of SlTCP12, SlTCP15 and SlTCP18, and that CNR binds the SlTCP18 promoter. This data strongly suggests that these class I SlTCP proteins are involved in ripening. Furthermore, we demonstrate that SlTCPs bind the promoter fragments of members of their own family, indicating that they regulate each other. Additional yeast one-hybrid studies performed with Arabidopsis transcription factors revealed binding of the promoter fragments by proteins involved in the ethylene signal transduction pathway, contributing to the idea that these SlTCP genes are involved in the ripening process. Yeast two-hybrid data shows that SlTCP proteins can form homo and heterodimers, suggesting that they act together in order to form functional protein complexes and together regulate developmental processes in tomato. Conclusions The comprehensive analysis we performed, like phylogenetic analysis, expression studies, identification of the upstream regulators and the dimerization specificity of the tomato TCP transcription factor family provides the basis for functional studies to reveal the role of this family in tomato development.
Databáze: OpenAIRE
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