In vivo visualization and ex vivo quantification of experimental myocardial infarction by indocyanine green fluorescence imaging
| Autor: | Dmitry Sonin, Dmitry Kurapeev, Nickolay Petrishchev, Michael M. Galagudza, Svetlana Chefu, S. M. Minasian, Jarle Vaage, Evgeniia Pochkaeva, G. V. Papayan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty Fluorophore business.industry Ischemia Vascular permeability 030204 cardiovascular system & hematology medicine.disease Atomic and Molecular Physics and Optics Extravasation Article Staining 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry In vivo 030220 oncology & carcinogenesis Medicine cardiovascular diseases business Nuclear medicine Indocyanine green Ex vivo Biotechnology |
| Popis: | The fluorophore indocyanine green accumulates in areas of ischemia-reperfusion injury due to an increase in vascular permeability and extravasation of the dye. The aim of the study was to validate an indocyanine green-based technique of in vivo visualization of myocardial infarction. A further aim was to quantify infarct size ex vivo and compare this technique with the standard triphenyltetrazolium chloride staining. Wistar rats were subjected to regional myocardial ischemia (30 minutes) followed by reperfusion (n = 7). Indocyanine green (0.25 mg/mL in 1 mL of normal saline) was infused intravenously for 10 minutes starting from the 25th minute of ischemia. Video registration in the near-infrared fluorescence was performed. Epicardial fluorescence of indocyanine green corresponded to the injured area after 30 minutes of reperfusion. Infarct size was similar when determined ex vivo using traditional triphenyltetrazolium chloride assay and indocyanine green fluorescent labeling. Intravital visualization of irreversible injury can be done directly by fluorescence on the surface of the heart. This technique may also be an alternative for ex vivo measurements of infarct size. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|