Plate ethanol-screening assay for selection of the Pichia stipitis and Hansenula polymorpha yeast mutants with altered capability for xylose alcoholic fermentation
| Autor: | Dorota Grabek-Lejko, Bernadetta Oklejewicz, Andriy Y. Voronovsky, Andriy A. Sibirny, Olena B. Ryabova |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiological Techniques
Xylose Ethanol biology Bioengineering Ethanol fermentation biology.organism_classification Applied Microbiology and Biotechnology Pichia Yeast Culture Media Mutagenesis Insertional chemistry.chemical_compound chemistry Biochemistry Xylose metabolism Fermentation Mutation Ethanol fuel Energy source Pichia stipitis Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology. 33:934-940 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-006-0147-7 |
| Popis: | A new method for the selection of Pichia stipitis and Hansenula polymorpha yeast mutants with altered capability to ferment xylose to ethanol was developed. The method is based on the ability of P. stipitis and H. polymorpha colonies to grow and produce ethanol on agar plates with xylose as the sole carbon and energy source. Secreted ethanol, in contrast to xylose, supports growth of cells of the indicator xylose-negative strains (the wild-type strain of Saccharomyces cerevisiae or Deltaxyl1 mutant of H. polymorpha) mixed with agar medium. The size of the tester culture-growth zone around xylose-grown colonies appeared to be dependent on the amount of secreted ethanol. Mutants with altered (decreased or elevated) ethanol production in xylose medium have been isolated using this method. The mutants exhibited pleiotropic alterations in enzymatic activities of the intermediary xylose metabolism. |
| Databáze: | OpenAIRE |
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