Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
| Autor: | Raimundo Cosme da Silva Filho, Jorge dos Santos Cavalcanti, Gorete Ribeiro de Macedo, Jackson Araújo de Oliveira, Everaldo Silvino dos Santos, Francine Ferreira Padilha, Sergio Carvalho de Santana |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Expanded Bed Adsorption
Chromatography biology Ion exchange Chemistry General Chemical Engineering Expanded bed adsorption Modeling Metarhizium anisopliae General Chemistry biology.organism_classification Modeling and simulation Hydrolysis Adsorption Mass transfer Chitosanase Dispersion (chemistry) Purification |
| Zdroj: | Repositório Institucional da UFRN Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
| Popis: | A mathematical model was developed to predict breakthrough curves during purification of the two chitosan- ases from Metarhizium anisopliae by expanded bed adsorption, taking into account the axial dispersion of liquid and using Streamline DEAE and SP XL adsorbents, anion and cation exchange resins, respectively. All the experiments were performed without clarification (with cells) aiming at the reduction of unit operations in future projects of separation processes, thereby reducing capital and operating costs. Chitosanases are enzymes that hydrolyze the carbohydrate chitosan, resulting in oligosaccharides that have many remarkable biological activities, such as anti-cancer, anti-HIV and antioxidant activities. The two adsorbents had similar performance in relation to hydrodynamics and mass transfer. The results of the parametric sensitivity analysis agree with the literature, and the model was validated with an average high degree of fit (94.68%) between simulated and experimental data obtained in this work |
| Databáze: | OpenAIRE |
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