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Additional file 1: Fig. S1. Transverse anther sections of Pyrgophyllum yunnanense at different developmental stages stained with Periodic acid - Schiff (PAS, a staining method used to detect polysaccharides) and Sudan Black B (a staining method used to detect lipid), showing the distribution of polysaccharides and lipids in different anther tissues. Polysaccharides stained red, lipid stained black dots. MMC, microspore mother cell; P, pollen grain; EMSP, early microspores; MSP, microspores; the arrow shows the liquid in the locule. Scale bars: 50μm. Fig. S2. Gene ontology (GO) classification of assembled unigenes of Caulokaempferia coenobialis (Cco), Hornstedtia hainanensis (Hhn), Pyrgophyllum yunnanense (Pyn), and Zingiber nudicarpum (Znu). Table S1. Summary of transcriptome data for Caulokaempferia coenobialis (Cco), Hornstedtia hainanensis (Hhn), Pyrgophyllum yunnanense (Pyn), and Zingiber nudicarpum (Znu). Table S2. Summary of functional annotation of unigenes of Caulokaempferia coenobialis (Cco), Hornstedtia hainanensis (Hhn), Pyrgophyllum yunnanense (Pyn), and Zingiber nudicarpum (Znu). Table S3. KOG classification of unigenes in Caulokaempferia coenobialis (Cco), Hornstedtia hainanensis (Hhn), Pyrgophyllum yunnanense (Pyn), and Zingiber nudicarpum (Znu).were classified into 25 functional categories. Table S4. KEGG_classification_count of Caulokaempferia coenobialis (Cco), Hornstedtia hainanensis (Hhn), Pyrgophyllum yunnanense (Pyn), and Zingiber nudicarpum (Znu). Table S5. Enriched GO Biological Process (GO BP) and KEGG Pathway of specific highly expressed genes in four gingers. Table S5-1. Enriched GO Biological Process (GO BP) and KEGG Pathway of specific highly expressed genes in Caulokaempferia coenobialis (Cco). Table S5-2. Enriched GO Biological Process (GO-BP) and KEGG Pathway of specific highly expressed genes in Hornstedtia hainanensis (Hhn). Table S5-3. Enriched GO Biological Process (GO-BP) and KEGG Pathway of specific highly expressed genes in Pyrgophyllum yunnanense (Pyn). Table S5-4. Enriched GO Biological Process (GO-BP) and KEGG Pathway of specific highly expressed genes in Zingiber nudicarpum (Znu). Table S6. Detection of selection for pollen coat formation genes in Caulokaempferia coenobialis (Cco), Hornstedtia hainanensis (Hhn) Branch using Branch Model of PAML. Table S8. Enriched GO Biological Process (GO-BP), and KEGG Pathway of positively selected genes in Caulokaempferia coenobialis (Cco) and Hornstedtia hainanensis (Hhn) branches. Table S8-1. Enriched GO Biological Process (GO-BP), and KEGG Pathway of positively selected genes in Caulokaempferia coenobialis (Cco) branch. Table S8-2. Enriched GO Biological Process (GO-BP), and KEGG Pathway of positively selected genes in Hornstedtia hainanensis (Hhn) branch. Table S9. Candidates genes of Caulokaempferia coenobialis and Hornstedtia hainanensis involved in mucilage-like or gum-like pollen coat formation. Table S9-1. Candidates' genes of Caulokaempferia coenobialis involved in mucilage-like pollen coat formation. Table S9-2. Candidates' genes of Hornstedtia hainanensis involved in mucilage-like pollen coat formation. Table S10. Expression pattern of some genes related to pollen wall formation in four gingers, Caulokaempferia coenobialis (Cco), Hornstedtia hainanensis (Hhn), Pyrgophyllum yunnanense (Pyn), Zingiber nudicarpum (Znu) and their homologs in Arabidopsis thaliana (At). |
