Molecular analysis of the influences of positive selection, tolerance induction, and antigen presentation on the T cell receptor repertoire
| Autor: | P J Fink, Stephen M. Hedrick, L A Matis, M J Blair |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Male
T cell T-Lymphocytes Immunology Antigen presentation Receptors Antigen T-Cell Antigen-Presenting Cells chemical and pharmacologic phenomena Cytochrome c Group Mice Inbred Strains Major histocompatibility complex Lymphocyte Activation Cell Line Major Histocompatibility Complex Mice medicine Immune Tolerance Immunology and Allergy Cytotoxic T cell Animals Selection Genetic Antigen-presenting cell Columbidae Gene Rearrangement biology Chimera T-cell receptor hemic and immune systems Articles MHC restriction Molecular biology Blotting Southern medicine.anatomical_structure Phenotype biology.protein Female Immunization CD8 |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Immunization of both B10.A and B10.S(9R) mice with pigeon cytochrome c (pcc) elicits T cells capable of proliferating to pcc presented on I-E major histocompatibility complex (MHC) molecules. The T cell receptor (TCR) repertoire used by pcc-specific T cells from these two strains is markedly different, even for T cells recognizing very similar antigen/MHC complexes. Our current studies have been directed toward explaining this differential expression between MHC congenic strains of TCR gene elements capable of recognizing similar ligands. Analysis of the TCR repertoire of pcc-specific T cells from F1[B10.A x B10.S (9R)]----parent radiation chimeras has demonstrated that much of this difference is a result of the positive selection of T cells for MHC restriction specificity. Further analysis of T cell lines from F1 mice and from radiation chimeras stimulated in vitro with pcc on both B10.A and B10.S(9R) antigen-presenting cells has provided clear-cut examples of the influence of positive selection, tolerance induction and of both in vivo and in vitro antigen presentation on the shaping of the TCR repertoire for a protein antigen. This is the first molecular analysis of how positive selection, tolerance induction, and antigen presentation can combine to mold the TCR repertoire. |
| Databáze: | OpenAIRE |
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