Integration of apoptosis and telomere erosion in virus-specific CD8+ T cells from blood and tonsils during primary infection
| Autor: | Joanne E. Cook, Maria V. D. Soares, Jeff M. Faint, Arne N. Akbar, W. Bergler, Jean M. Fletcher, Peter C. L. Beverley, Nicholas Hammerschmitt, Fiona J. Plunkett, Malcolm H.A. Rustin, Mike Salmon, Lavina L. Belaramani, Caroline S. Verbeke |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Herpesvirus 4
Human Telomerase Palatine Tonsil Immunology Apoptosis CD8-Positive T-Lymphocytes Biology Biochemistry Interleukin 21 Immune system CD28 Antigens Humans Cytotoxic T cell Infectious Mononucleosis Interleukin 3 CD28 Cell Differentiation Cell Biology Hematology Molecular biology Telomere Case-Control Studies Acute Disease Cytokines Immunologic Memory Cell Division CD8 |
| DOI: | 10.1182/blood-2003-06-1791 |
| Popis: | Human-virus-specific CD8+ T cells that are found during primary infection have been studied almost exclusively in the peripheral blood, and it is unclear whether these cells are regulated in the same way as those in secondary lymphoid tissue. We investigated, therefore, the control of apoptosis and telomere erosion of Epstein-Barr virus (EBV)-specific CD8+ T cells found in the blood and tonsils of the same patients during acute infectious mononucleosis (AIM). Although the clonal composition of CD8+ T cells as determined by heteroduplex analysis was similar in both compartments, there was greater CD28 expression in the tonsil population, indicating that they were less differentiated. EBV-specific CD8+ T cells in both tissue types were extremely susceptible to apoptosis related to low Bcl-2 expression and were dependent on exogenous cytokines such as interleukin-2 (IL-2), IL-15, and interferon-alpha/beta (IFN-alpha/beta) for survival. In both compartments, however, these cells maintained their telomere lengths through telomerase induction. Thus, apoptosis-prone EBV-specific CD8+ T cells found during acute infection have to be rescued from death to persist as a memory population. However, signals that induce telomerase ensure that the rescued cells retain their replicative capacity. Significantly, these processes operate identically in cells found in blood and secondary lymphoid tissue. |
| Databáze: | OpenAIRE |
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