Analyses of bovine luteal fractions obtained by FACS reveals enrichment of miR-183-96-182 cluster miRNAs in endothelial cells
Autor: | Cristina L. Esteves, Bushra T Mohammed, F. Xavier Donadeu |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Cell Nile Red Corpus luteum 0302 clinical medicine Endocrinology lcsh:Reproduction 030219 obstetrics & reproductive medicine Chemistry bovine Nile red Obstetrics and Gynecology Bovine Cell sorting Flow Cytometry steroidogenic Steroidogenic medicine.anatomical_structure Real-time polymerase chain reaction miRNAs Female miR-183-96-182 Cell type lcsh:QH471-489 Short Communication CD146 Antigen Luteal phase Real-Time Polymerase Chain Reaction lcsh:Gynecology and obstetrics Endothelial 03 medical and health sciences endothelial microRNA medicine Animals luteal cells lcsh:RG1-991 copus luteum Endothelial Cells Molecular biology MicroRNAs Luteal cells 030104 developmental biology Reproductive Medicine HSD3B1 miR-212-132 Cattle Biomarkers Developmental Biology |
Zdroj: | Reproductive Biology and Endocrinology, Vol 17, Iss 1, Pp 1-6 (2019) Reproductive Biology and Endocrinology : RB&E Mohammed, B, Esteves, C & Donadeu, F 2019, ' Analyses of bovine luteal fractions obtained by FACS reveals enrichment of miR-183-96-182 cluster miRNAs in endothelial cells ', Reproductive Biology and Endocrinology, vol. 17, 41 . https://doi.org/10.1186/s12958-019-0484-9 |
ISSN: | 1477-7827 |
Popis: | Our previous studies showed that the miRNA clusters, miR-183-96-182 and miR-212-132, may be critical in promoting luteal cell survival and progesterone production in both bovine and humans. To further understand their involvement in luteal development, this study aimed to establish the expression of these miRNAs in different bovine luteal cell types, namely, endothelial and steroidogenic, isolated using fluorescence-activated cell sorting (FACS). We isolated each of the two cell populations based on the presence of the endothelia surface marker, CD144, and uptake of the lipophilic dye, Nile Red, respectively. Using quantitative Polymerase Chain Reaction (qPCR) in the sorted cell fractions we confirmed that CD144 and the endothelia-specific miRNA, miR-126, were predominantly expressed in endothelial cells (CD144+), whereas HSD3B1 was expressed predominantly in steroidogenic cells (Nile RedHI). Finally, we found that whereas the miR-212-132 cluster was expressed at similar levels in luteal endothelial and steroidogenic cells, miR-183-96-182 was expressed at > 4-fold higher levels in endothelial than in steroidogenic cells (P |
Databáze: | OpenAIRE |
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