Differences in reproductive toxicity of TBBPA and TCBPA exposure in male Rana nigromaculata
| Autor: | Xiaodong Shan, Ning Li, Hangjun Zhang, Jianbo He, Feifei Chen, Xiuying Jia, Qiongxia Du, Juan Tang, Bin Chen, Wenli Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Ranidae Health Toxicology and Mutagenesis Polybrominated Biphenyls 010501 environmental sciences Toxicology 01 natural sciences 03 medical and health sciences Follicle-stimulating hormone chemistry.chemical_compound Internal medicine medicine Animals Testosterone Spermatogenesis 0105 earth and related environmental sciences Dose-Response Relationship Drug Estradiol Sperm Count Chemistry Reproduction General Medicine Luteinizing Hormone Pollution Sperm Spermatozoa 030104 developmental biology Endocrinology Receptors Androgen Toxicity Sperm Motility Tetrabromobisphenol A Follicle Stimulating Hormone Reproductive toxicity Luteinizing hormone Chlorophenols |
| Zdroj: | Environmental pollution (Barking, Essex : 1987). 243 |
| ISSN: | 1873-6424 |
| Popis: | Tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol A (TCBPA) are persistent toxic environmental pollutants that cause severe reproductive toxicity in animals. The goal of this study was to compare the reproductive toxic effects of TBBPA and TCBPA on male Rana nigromaculata and to expound on the mechanisms leading to these effects. Healthy adult frogs were exposed to 0, 0.001, 0.01, 0.1, and 1 mg/L of TBBPA and TCBPA for 14 days. Sperm numbers were counted by erythrometry. Sperm mobility and deformities were observed under a light microscope (400 × ). We used commercial ELISA kits to determine the serum content of testosterone (T), estradiol (E2), luteinizing hormone (LH) and follicle stimulating hormone (FSH). Expression of androgen receptor (AR) mRNA was detected using real-time qPCR. Sperm numbers and sperm mobility were significantly decreased and sperm deformity was significantly increased in a concentration dependent manner following exposure to TBBPA and TCBPA. Sperm deformity was significantly greater in the 1 mg/L TCBPA (0.549) treatment group than in the 1 mg/L TBBPA (0.397) treatment group. Serum T content was significantly greater in the 0.01, 0.1 and 1 mg/L TBBPA and TCBPA experimental groups compared with controls, while E2 content was significantly greater in only the 1 mg/L TBBPA and TCBPA experimental groups. Expression levels of LH and FSH significantly decreased in the 1 mg/L TBBPA and TCBPA treatment groups. AR mRNA expression decreased markedly in all the treated groups. Our results indicated that TBBPA and TCBPA induced reproductive toxicity in a dose-dependent manner, with TCBPA having greater toxicity than TBBPA. Furthermore, changes in T, E2, LH, and FSH levels induced by TBBPA and TCBPA exposure, which led to endocrine disorders, also caused disturbance of spermatogenesis through abnormal gene expressions of AR in the testes. |
| Databáze: | OpenAIRE |
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