Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis
| Autor: | Bofeng Zhu, Li Wang, Shi‑Xiu Liao, Qian‑Nan Guo, Lin Liu, Qiao‑Fang Hou, Jing‑Bin Yan, Hui‑Ru Zhao, Wei‑Li Shi, Hong‑Dan Wang |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Epigenomics 0301 basic medicine Cancer Research Gestational Age Prenatal diagnosis Biology Biochemistry Epigenesis Genetic 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy Prenatal Diagnosis Genetics Humans Cancer epigenetics Promoter Regions Genetic Molecular Biology Gene Oligonucleotide Array Sequence Analysis DNA methylation 030219 obstetrics & reproductive medicine bisulfate direct sequencing Gene Expression Profiling Computational Biology Sequence Analysis DNA Articles Methylation 030104 developmental biology Oncology chemistry CpG site Molecular Medicine CpG Islands Female Biomarkers GeneChip human tiling 2.0R array set DNA Genome-Wide Association Study |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2017.6506 |
| Popis: | The discovery of cell-free DNA fetal (cff DNA) in maternal plasma during pregnancy provides a novel perspective for the development of non-invasive prenatal diagnosis (NIPD). Against the background of maternal DNA, the use of the relatively low concentration of cff DNA is limited in NIPD. Therefore, in order to overcome the complication of the background of maternal DNA and expand the scope of cff DNA application in clinical practice, it is necessary to identify novel universal fetal-specific DNA markers. The GeneChip Human Promoter 1.0R Array set was used in the present study to analyze the methylation status of 12 placental tissue and maternal peripheral blood whole-genome DNA samples. In total, 5 fetus differential hypermethylation regions and 6 fetus differential hypomethylation regions were identified. In order to verify the 11 selected methylation regions and detect the differential CpG sites in these regions, a bisulfate direct sequencing strategy was used. In total, 87 fetal differential methylation CpG sites were identified from 123 CpG sites. The detection of fetal differential methylation DNA regions and CpG sites may be instrumental in the development of efficient NIPD and in the expansion of its application in other disorders. |
| Databáze: | OpenAIRE |
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