Effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine
| Autor: | Chaitanya G. Joshi, Linz-Buoy George, Nidhi P. Raval, Tejas M. Shah |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
endocrine system
droplet digital polymerase chain reaction Offspring Veterinary medicine Y chromosome SF1-1100 Andrology 03 medical and health sciences 0302 clinical medicine Human fertilization spermatozoa SF600-1100 swim-up X chromosome 030219 obstetrics & reproductive medicine General Veterinary urogenital system Chemistry Hybridization probe 0402 animal and dairy science Chromosome 04 agricultural and veterinary sciences 040201 dairy & animal science Sperm Animal culture Research Article |
| Zdroj: | Veterinary World Veterinary World, Vol 12, Iss 8, Pp 1299-1303 (2019) |
| ISSN: | 2231-0916 0972-8988 |
| DOI: | 10.14202/vetworld.2019.1299-1303 |
| Popis: | Background and Aim: Studies have shown that the pH of the vagina during the course of fertilization may influence the migration of X- and Y-bearing spermatozoa and thus leading to skewness in the sex of the offspring. Hence, this study was carried out to check the effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine (Bos indicus). Materials and Methods: To check the effect of pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine, we used buffers of various pH ranging from 5.5 to 9.0 for swim-up procedure of sperm sample and collected upper and bottom fraction from the same buffer and checked the abundance of X- and Y-bearing spermatozoa by droplet digital polymerase chain reaction using X- and Y-chromosome-specific DNA probe. Results: The abundance of X- and Y-bearing spermatozoa was not differed significantly in either of the fraction collected. Conclusion: Thus, it appears to be unlikely that an immediate impact of pH on sperm can be a solitary impact on the sex of offspring in bovine. Keywords: droplet digital polymerase chain reaction, spermatozoa, swim-up. |
| Databáze: | OpenAIRE |
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