CHIR99021 balance TGFβ1 induced human corneal endothelial-to-mesenchymal transition to favor corneal endothelial cell proliferation

Autor:	Yiran Wang, Caixia Jin, Haibin Tian, Jingying Xu, Jie Chen, Shuqin Hu, Qian Li, Lixia Lu, Qingjian Ou, Guo-tong Xu, Hongping Cui
Rok vydání:	2022
Předmět:	Adult Transforming Growth Factor beta1 Cellular and Molecular Neuroscience Ophthalmology Epithelial-Mesenchymal Transition Pyrimidines Pyridines Endothelium Corneal Endothelial Cells Humans Cells Cultured Sensory Systems Cell Proliferation
Zdroj:	Experimental Eye Research. 219:108939
ISSN:	0014-4835
Popis:	Corneal endothelial cells (CECs) play a major role in the maintenance of stromal hydration via the barrier and pump function for clear vision. Adult CECs cannot regenerate after injury. CECs cultured in vitro can undergo mitosis but may undergo corneal endothelial-to-mesenchymal transition (EnMT) and lose their endothelial characteristics. In this study, we examined the effects of CHIR99021 on transforming growth factor beta-1(TGFβ1)-induced EnMT in human CECs (hCECs) lines. CHIR99021 kept hCECs in the hexagonal shape and could downregulate the EnMT markers alpha-smooth muscle actin (α-SMA) and fibronectin (FN1), meanwhile maintained the hCECs function markers Na
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16c072a21dad557079cb14217b858fa9 https://doi.org/10.1016/j.exer.2022.108939 Zobrazit plný text záznamu Full Text from ScienceDirect