Bioreactor‐manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies
| Autor: | Lauren Hails, Graziella Pellegrini, Ivan Martin, Maja Pušić, Helen L. Quasnichka, Alan Ivković, Timothy Smith, Christian Krause, Maria Adelaide Asnaghi, Andreja Vukasović, David Wendt, Anke Wixmerten, Elisa Figallo, Wael Kafienah, Petar Kostešić, Giuseppe Filardo, Damir Hudetz, Elizaveta Kon, Nuala Trainor, Drazen Maticic, Carmine Cozzolino |
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| Rok vydání: | 2019 |
| Předmět: |
Cartilage
Articular 0301 basic medicine Scaffold large animal study Bioreactor Cartilage tissue engineering bioreactor 03 medical and health sciences Bioreactors Chondrocytes cartilage repair manufacturing osteochondral tissue engineering Acute Disease Animals Chronic Disease Female Models Animal Sheep Tissue Engineering Tissue Scaffolds 0302 clinical medicine Tissue engineering bioreactor cartilage repair large animal study manufacturing osteochondral tissue engineering medicine Human cartilage business.industry Cartilage technology industry and agriculture Original Articles Cell Biology General Medicine equipment and supplies 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Original Article Manufacturing methods business Large animal Biomedical engineering |
| Zdroj: | Vukasovic, A, Asnaghi, M A, Kostesic, P, Quasnichka, H L, Cozzolino, C, Pusic, M, Hails, L, Trainor, N, Krause, C, Figallo, E, Filardo, G, Kon, E, Wixmerten, A, Maticic, D, Pellegrini, G, Kafienah, W, Hudetz, D, Smith, T, Martin, I, Ivkovic, A & Wendt, D 2019, ' Bioreactor manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies ', Cell Proliferation . https://doi.org/10.1111/cpr.12653 Cell Proliferation |
| ISSN: | 1365-2184 0960-7722 |
| Popis: | Objectives Bioreactor-based production systems have the potential to overcome limitations associated with conventional tissue engineering manufacturing methods, facilitating regulatory compliant and cost-effective production of engineered grafts for widespread clinical use. In this work, we established a bioreactor-based manufacturing system for the production of cartilage grafts. Materials & methods All bioprocesses, from cartilage biopsy digestion through the generation of engineered grafts, were performed in our bioreactor-based manufacturing system. All bioreactor technologies and cartilage tissue engineering bioprocesses were transferred to an independent GMP facility, where engineered grafts were manufactured for two large animal studies. Results The results of these studies demonstrate the safety and feasibility of the bioreactor-based manufacturing approach. Moreover, grafts produced in the manufacturing system were first shown to accelerate the repair of acute osteochondral defects, compared to cell-free scaffold implants. We then demonstrated that grafts produced in the system also facilitated faster repair in a more clinically relevant chronic defect model. Our data also suggested that bioreactor-manufactured grafts may result in a more robust repair in the longer term. Conclusion By demonstrating the safety and efficacy of bioreactor-generated grafts in two large animal models, this work represents a pivotal step towards implementing the bioreactor-based manufacturing system for the production of human cartilage grafts for clinical applications. Read the Editorial for this article on doi:10.1111/cpr.12625. |
| Databáze: | OpenAIRE |
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