Comparative analyses of multi-species sequences from targeted genomic regions
| Autor: | Stephen M. Beckstrom-Sternberg, Donna Karolchik, Baoli Zhu, M. C. Huang, Tyrone J. Summers, J. T. Tran, Jeffrey W. Touchman, Jacquelyn R. Idol, L. S. Wiggins, James W. Thomas, Charles P. Brinkley, N. Akhter, Scott Schwartz, M. S. Schwartz, Laura Elnitski, Nancy F. Hansen, Eric D. Green, David J. Cutler, Quino Maduro, Aravinda Chakravarti, Mathieu Blanchette, K. Ayele, Robert W. Blakesley, J. C. McCloskey, Sirintorn Stantripop, Shih-Queen Lee-Lin, Keith Wetherby, Elliott H. Margulies, Alice C. Young, Nicole Dietrich, M. A. Walker, Stephen D. Mastrian, P. L. Laric, Jenny McDowell, Richelle Legaspi, B. Zhao, S. L. Ho, Webb Miller, R. Bevan, Pamela J. Thomas, Philip Green, Kazutoyo Osoegawa, Emmanuelle Tiongson, Betty Benjamin, S. Granite, P. Haghighi, L. H. Zhang, Cathy Masiello, Arian F.A. Smit, Baishali Maskeri, Jennifer Vogt, T. C. Bruen, W. J. Kent, David Haussler, Eric Karlins, P. J. De Jong, Matthew E. Portnoy, R. J. Weber, C. L. Shu, C. Tsurgeon, Xin-Yuan Guan, R. Pearson, K. Cariaga, Shelise Brooks, Gerry Bouffard, Arjun B. Prasad, Adam Siepel, Charles E. Lawrence, Jyoti Gupta, M. J. Lim, Valerie Maduro |
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| Rok vydání: | 2003 |
| Předmět: |
Transposable element
Cystic Fibrosis Transmembrane Conductance Regulator Genomics Genome Evolution Molecular Species Specificity biology.animal Sequence Homology Nucleic Acid Animals Humans Gene Conserved Sequence Phylogeny Sequence (medicine) Comparative genomics Genetics Mammals Multidisciplinary biology Vertebrate Sister group Evolutionary biology Mutagenesis Vertebrates DNA Transposable Elements Sequence Alignment Chromosomes Human Pair 7 |
| Zdroj: | Nature. 424(6950) |
| ISSN: | 1476-4687 |
| Popis: | The systematic comparison of genomic sequences from different organisms represents a central focus of contemporary genome analysis. Comparative analyses of vertebrate sequences can identify coding1,2,3,4,5,6 and conserved non-coding4,6,7 regions, including regulatory elements8,9,10, and provide insight into the forces that have rendered modern-day genomes6. As a complement to whole-genome sequencing efforts3,5,6, we are sequencing and comparing targeted genomic regions in multiple, evolutionarily diverse vertebrates. Here we report the generation and analysis of over 12 megabases (Mb) of sequence from 12 species, all derived from the genomic region orthologous to a segment of about 1.8 Mb on human chromosome 7 containing ten genes, including the gene mutated in cystic fibrosis. These sequences show conservation reflecting both functional constraints and the neutral mutational events that shaped this genomic region. In particular, we identify substantial numbers of conserved non-coding segments beyond those previously identified experimentally, most of which are not detectable by pair-wise sequence comparisons alone. Analysis of transposable element insertions highlights the variation in genome dynamics among these species and confirms the placement of rodents as a sister group to the primates. |
| Databáze: | OpenAIRE |
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