The Transcriptional Effect of WT1 Is Modulated by Choice of Expression Vector
| Autor: | Josina C. Reddy, Seiyu Hosono, Jonathan D. Licht |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Genes Wilms Tumor Transcription Genetic Genetic Vectors Response element Cytomegalovirus Gene Expression Repressor Biology Kidney Transfection urologic and male genital diseases Biochemistry Cell Line Transcription (biology) Chlorocebus aethiops Animals Promoter Regions Genetic WT1 Proteins Molecular Biology Transcription factor Repetitive Sequences Nucleic Acid Zinc finger transcription factor Expression vector urogenital system Activator (genetics) fungi Zinc Fingers Promoter Cell Biology Molecular biology female genital diseases and pregnancy complications DNA-Binding Proteins Kinetics Avian Sarcoma Viruses Transcription Factors |
| Zdroj: | Journal of Biological Chemistry. 270:29976-29982 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.270.50.29976 |
| Popis: | The WT1 Wilms' tumor suppressor gene encodes a zinc finger transcription factor which plays a critical role in renal and genitourinary development. The WT1 protein was reported to both activate and repress transcription. We found that the transcriptional effect of WT1 on the Egr1 promoter could be modulated by the use of expression vectors containing different promoters. WT1 activated the Egr1 promoter when expression of WT1 was driven by the Rous sarcoma virus promoter. In contrast, a cytomegalovirus (CMV) promoter-containing WT1 expression vector repressed the Egr1 promoter. However, WT1 activated transcription of a simple test promoter, EGR3tkCAT, regardless of the expression vector used. Co-transfection of the parental CMV-based vector strongly depressed the basal activity of the Egr1-CAT reporter, suggesting that the CMV promoter competes with the Egr1 promoter for transcription factors or co-factors which may be required for activation by WT1. In support of this hypothesis, WT1 was converted from an activator to a repressor by co-transfection of an excess of the parental CMV-based vector. These results provide an important caveat to the interpretation of co-transfection studies and confirm the bi-functional nature of the WT1 transcription factor. |
| Databáze: | OpenAIRE |
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