Alpha lipoic acid supplementation attenuates reactive oxygen species in hypothalamic paraventricular nucleus and sympathoexcitation in high salt-induced hypertension
| Autor: | Yu-Ming Kang, Jin-Jun Liu, Hong-Bao Li, Chan-Juan Huo, Wei Cui, Yu-Wang Miao, Qing Yang, Meng Zhang, Jing Guo, Qing Su, Xiao-Lian Shi |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Mean arterial pressure Sympathetic Nervous System 030204 cardiovascular system & hematology Kidney Toxicology medicine.disease_cause Antioxidants Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Arterial Pressure Rats Wistar Sodium Chloride Dietary chemistry.chemical_classification Reactive oxygen species Thioctic Acid biology Superoxide General Medicine Angiotensin II Rats 030104 developmental biology medicine.anatomical_structure Blood pressure Endocrinology chemistry Hypertension biology.protein Reactive Oxygen Species Oxidative stress Paraventricular Hypothalamic Nucleus |
| Zdroj: | Toxicology Letters. 241:152-158 |
| ISSN: | 0378-4274 |
| Popis: | Aims High salt-induced oxidative stress plays an important role in the development of hypertension. Alpha lipoic acid (ALA) is extensively recognized as having a powerful superoxide inhibitory property. In this study, we determined whether ALA supplementation attenuates oxidative stress in hypothalamic paraventricular nucleus (PVN), decreases the sympathetic activity and arterial pressure in high salt-induced hypertension by cross-talking with renin–angiotensin system (RAS) and pro-inflammatory cytokines (PICs). Methods Male Wistar rats were administered a normal-salt diet (NS, 0.3% NaCl) or a high-salt diet (HS, 8.0% NaCl) for 8 weeks. These rats received ALA (60 mg/kg) dissolved in vehicle (0.9% saline) or an equal voleme of vehicle, by gastric perfusion for 9 weeks. Results High salt intake resulted in higher renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP). These rats also had higher levels of superoxide, gp91 phox , gp47 phox (subunits of NAD(P)H oxidase), angiotensin-converting enzyme (ACE), angiotensin II type1 receptor (AT1-R), interleukin-1beta (IL-1β), interleukin-6 (IL-6), and lower levels of interleukin-10 (IL-10) and copper/zinc superoxide dismutase (Cu/Zn-SOD) than control animals. Treatment with ALA significantly attenuated the levels of superoxide, gp91 phox , gp47 phox , ACE, AT1-R, IL-1β and IL-6, increased the levels of IL-10 and Cu/Zn-SOD, and decreased MAP and RSNA compared with high-salt induced hypertensive rats. The mRNA expression of gp47 phox and gp91 phox are in accordance with their protein expression. Conclusion These findings suggest that supplementation of ALA obviously decreases the sympathetic activity and arterial pressure in high salt-induced hypertension by improving the superoxide inhibitory property, suppressing the activation of RAS and restoring the balance between pro- and anti-inflammatory cytokines in the PVN. |
| Databáze: | OpenAIRE |
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