Pharmacological and transcriptomic characterization of the nitric oxide pathway in aortic rings isolated from the tortoise Chelonoidis carbonaria
| Autor: | Felipe Fernandes Jacintho, José Carlos Cogo, Rafael Campos, Julio Alejandro Rojas-Moscoso, Mauro Napolitano, Gilberto De Nucci, Ronilson Agnaldo Moreno, Fabíola Z. Mónica, Alberto Fernando Oliveira Justo |
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| Rok vydání: | 2019 |
| Předmět: |
030110 physiology
0301 basic medicine Male Physiology Health Toxicology and Mutagenesis Pharmacology Toxicology Nitric Oxide Biochemistry Nitric oxide Transcriptome 03 medical and health sciences chemistry.chemical_compound Soluble Guanylyl Cyclase medicine.artery medicine Animals HISTAMINA Aorta Cyclic Nucleotide Phosphodiesterases Type 5 biology Activator (genetics) Brain Cell Biology General Medicine biology.organism_classification Adenosine Turtles 030104 developmental biology Chelonoidis chemistry Gene Expression Regulation Purinergic Agonists Vasoconstriction Nitric Oxide Pathway cardiovascular system Female Histamine medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1532-0456 |
| Popis: | In this study the nitric oxide (NO)-soluble guanylate cyclase (sGC) and phosphodiesterase-5 (PDE-5) pathway was characterized in tortoise Chelonoidis carbonaria aorta. Concentration response curves (CCR) to ATP, ADP, AMP, adenosine and histamine were performed in the presence and absence of L-NAME in aorta pre-contracted with ACh (3 μM). CCR to SNP, BAY 41-2272 (sGC stimulator), BAY 60-2770 (sGC activator) and tadalafil (PDE-5 inhibitor) were constructed in the presence and absence of ODQ (10 μM). ATP (pEC50 6.1 ± 0.1), ADP (pEC50 6.0 ± 0.2), AMP (pEC50 6.8 ± 0.1) and histamine (pEC50 6.8 ± 0.12) relaxed Chelonoidis aorta and the addition of L-NAME reduced their efficacy (p |
| Databáze: | OpenAIRE |
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