Quantitative PCR tissue expression profiling of the human SGLT2 gene and related family members
| Autor: | Howard Loraine, John N. Feder, Jean M. Whaley, Samantha Ho, Deborah Hagan, Jian Chen, Sandy Williams |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Kidney
urogenital system Endocrinology Diabetes and Metabolism Glucose transporter Skeletal muscle Biology SGLT2 Renal glucose reabsorption tissue expression medicine.anatomical_structure Real-time polymerase chain reaction Biochemistry quantitative PCR Internal Medicine medicine sodium-glucose cotransporter protein type 2 diabetes Amino acid transporter Cotransporter Gene Original Research |
| Zdroj: | Diabetes Therapy |
| ISSN: | 1869-6961 1869-6953 |
| Popis: | SGLT2 (for “Sodium GLucose coTransporter” protein 2) is the major protein responsible for glucose reabsorption in the kidney and its inhibition has been the focus of drug discovery efforts to treat type 2 diabetes. In order to better clarify the human tissue distribution of expression of SGLT2 and related members of this cotransporter class, we performed TaqMan™ (Applied Biosystems, Foster City, CA, USA) quantitative polymerase chain reaction (PCR) analysis of SGLT2 and other sodium/glucose transporter genes on RNAs from 72 normal tissues from three different individuals. We consistently observe that SGLT2 is highly kidney specific while SGLT5 is highly kidney abundant; SGLT1, sodium-dependent amino acid transporter (SAAT1), and SGLT4 are highly abundant in small intestine and skeletal muscle; SGLT6 is expressed in the central nervous system; and sodium myoinositol cotransporter is ubiquitously expressed across all human tissues. Electronic Supplementary Material Supplementary material is available for this article at 10.1007/s13300-010-0006-4 and is accessible for authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink