A novel device to measure static hindlimb weight-bearing forces in pronograde rodents
| Autor: | Juan Valdivia, Rachel C. Meyers, B. Duncan X. Lascelles, Michael W. Nolan, Samantha L. Sommer, Morika D. Williams |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Analgesic Posture Plantar surface Pain Hindlimb medicine.disease_cause Article Weight-bearing Weight-Bearing 03 medical and health sciences Mice 0302 clinical medicine Medicine Animals Limb loading Inflammation Behavior Animal business.industry General Neuroscience Neurosciences Inflammatory pain Biomechanical Phenomena Rats Meloxicam Disease Models Animal 030104 developmental biology Anesthesia Joint pain medicine.symptom business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | J Neurosci Methods |
| ISSN: | 1872-678X |
| Popis: | Background Joint pain is composed of both spontaneous and movement-induced pain. In animal models, static bodyweight distribution is a surrogate for spontaneous joint pain. However, there are no commercially-available instruments that measure static bodyweight distribution in normal, pronograde rodents. New method We designed a Static Horizontal Incapacitance Meter (SHIM) to measure bodyweight distribution in pronograde standing rodents. We assessed the device for feasibility, repeatability, and sensitivity to quantify hindlimb bodyweight distribution. Mice and rats with unilateral inflammatory pain induced by subcutaneous injections of capsaicin or Complete Freund’s Adjuvant (CFA) into the plantar surface of the left hind paw were used to measure static weight-bearing. The ability to attenuate inflammatory pain-associated weight-bearing asymmetry was tested by administering a non-steroidal anti-inflammatory drug, meloxicam. Results The SHIM’s ability to detect significant reductions in limb loading on the injected hindlimb in mice and rats was validated using both acute and sub-chronic pain models. Treatment with meloxicam partially reversed CFA-induced effects. Comparison with existing methods In contrast with assays that measure kinetic or static weight-bearing forces (e.g., walking, or standing at a 45 ° incline), the SHIM allows evaluation of weight-bearing in rodents that are standing at rest in their normal pronograde position. Conclusions The SHIM successfully detected: (a) asymmetric weight-bearing in acute and sub-chronic pain models; and (b) the analgesic effects of meloxicam. This study provides a novel tool to objectively evaluate limb use dysfunction in rodents. |
| Databáze: | OpenAIRE |
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