ADP-induced platelet aggregation and thrombin generation are increased in Essential Thrombocythemia and Polycythemia Vera
| Autor: | Anna Falanga, Carmen J Tartari, Marina Marchetti, Laura Russo, Hugo ten Cate, Anna Leuzzi, Guido Finazzi, Marina Panova-Noeva, Alessandro Rambaldi |
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| Přispěvatelé: | Biochemie, Interne Geneeskunde, RS: CARIM School for Cardiovascular Diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Blood Platelets Male aspirin Pharmacology Young Adult chemistry.chemical_compound Polycythemia vera Annexin JAK2617F mutation medicine Humans Platelet Polycythemia Vera thrombosis Aged Whole blood Aged 80 and over Aspirin Essential thrombocythemia business.industry Thrombin Hematology Janus Kinase 2 Middle Aged medicine.disease Thrombosis Adenosine Diphosphate myeloproliferative disorders chemistry platelet aggregation thrombin generation Mutation Immunology Female Arachidonic acid business Platelet Aggregation Inhibitors Thrombocythemia Essential medicine.drug |
| Zdroj: | Thrombosis Research, 132(1), 88-93. Elsevier Science |
| ISSN: | 1879-2472 0049-3848 |
| Popis: | Introduction Essential Thrombocythemia (ET) and Polycythemia Vera (PV) patients are characterized by an increased rate of thrombotic complications and by several abnormalities of platelets, more pronounced in JAK2V617F positive patients. The aim of this study was to characterize the platelet aggregation as well as the platelet procoagulant potential induced by several different agonists in ET and PV patients. Materials and Methods Venous blood samples were obtained from 65 ET and 51 PV patients. Whole blood impedance aggregometry was utilized to characterize platelet aggregation induced by collagen, ADP, thrombin receptor activating peptide and arachidonic acid, while the Calibrated Automated Thrombogram (CAT) assay was used to determine the thrombin generation (TG) potential induced by ADP in platelet-rich plasma. CAT assay was also performed in the presence of annexin V to evaluate the contribution of platelet phospholipids to TG. Results and Conclusions ADP-induced platelet aggregation and TG were significantly increased in ET and PV patients compared to controls. The highest values were observed in JAK2V617F positive patients and in patients on aspirin. In these subjects, annexin V was less effective in inhibiting both basal and ADP-induced TG. This study demonstrates for first time that platelets from ET and PV patients are more responsive to the ADP stimulus, in terms of both increased platelet aggregation, and enhanced TG, particularly in the JAK2V617F positive patients. Our data support the hypothesis that the use of ADP receptor inhibitors, in addition to aspirin, might be considered in the prevention of thrombosis in these conditions, by allowing a more complete inhibition of platelet functions. |
| Databáze: | OpenAIRE |
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