Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression
| Autor: | Yu Zou, Jia Liu, Liu-Lin Xiong, Xiaosong Hu, Chao-Zhi Luo, Qin-qin He, Yan-ping Wang, Ting-Hua Wang, Qing-Jie Xia, Xiang He |
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| Jazyk: | angličtina |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Cognitive Neuroscience Ischemia Lung injury Mesenchymal Stem Cell Transplantation Brain Ischemia Rats Sprague-Dawley Brain ischemia Random Allocation Bone marrow mesenchymal stem cells 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine stomatognathic system Acute lung injury Animals Medicine Biological Psychiatry Lung Tumor Necrosis Factor-alpha business.industry Research Mesenchymal stem cell Infarction Middle Cerebral Artery Mesenchymal Stem Cells Lung Injury General Medicine respiratory system medicine.disease Pulmonary edema Rats respiratory tract diseases Transplantation 030104 developmental biology medicine.anatomical_structure TNF-α Bone marrow business 030217 neurology & neurosurgery |
| Zdroj: | Behavioral and Brain Functions : BBF |
| ISSN: | 1744-9081 |
| DOI: | 10.1186/s12993-016-0093-0 |
| Popis: | Background Bone marrow mesenchymal stem cell (BMSCs)-based therapy seems to be a promising treatment for acute lung injury, but the therapeutic effects of BMSCs transplantation on acute lung injury induced by brain ischemia and the mechanisms have not been totally elucidated. This study explores the effects of transplantation of BMSCs on acute lung injury induced by focal cerebral ischemia and investigates the underlying mechanism. Methods Acute lung injury model was induced by middle cerebral artery occlusion (MCAO). BMSCs (with concentration of 1 × 106/ml) were transplanted into host through tail vein 1 day after MCAO. Then, the survival, proliferation and migration of BMSCs in lung were observed at 4 days after transplantation, and histology observation and lung function were assessed for 7 days. Meanwhile, in situ hybridization (ISH), qRT-PCR and western blotting were employed to detect the expression of TNF-α in lung. Results Neurobehavioral deficits and acute lung injury could be seen in brain ischemia rats. Implanted BMSCs could survive in the lung, and relieve pulmonary edema, improve lung function, as well as down regulate TNF-α expression. Conclusions The grafted BMSCs can survive and migrate widespread in lung and ameliorate lung injury induced by focal cerebral ischemia in the MCAO rat models. The underlying molecular mechanism, at least partially, is related to the suppression of TNF-α. |
| Databáze: | OpenAIRE |
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