Molecular mechanism of vde-initiated intein homing in yeast nuclear genome
| Autor: | Yuri Nagai, Yoshikazu Ohya, Tomoyuki Fukuda |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cytoplasm
Premeiotic DNA replication DNA Repair Saccharomyces cerevisiae Biophysics Biochemistry Models Biological Homing endonuclease Recognition sequence Protein splicing DNA Fungal Genetics Cell Nucleus biology Models Genetic Biological Transport DNA biology.organism_classification Cell biology Protein Structure Tertiary Kinetics Meiosis biology.protein Homologous recombination Intein Homing (hematopoietic) Signal Transduction |
| Zdroj: | Advances in biophysics. 38 |
| ISSN: | 0065-227X |
| Popis: | Summary In Saccharomyces cerevisiae, VMAI intein encodes a homing endonuclease termed VDE which is produced by an autocatalytic protein splicing reaction. VDE introduces a DSB at its recognition sequence on intein-minus allele, resulting in the lateral transfer of VMAI intein. In this review, we summarize a decade of in vitro study on VDE and describe our recent study on the in vivo behavior of both VDE and host proteins involved in intein mobility. Meiotic DSBs caused by VDE are repaired in the similar pathway to that working in meiotic recombination induced by Spollp-mediated DSBs. Meiosis-specific DNA cleavage and homing is shown to be guaranteed by the two distinct mechanisms, the subcellular localization of VDE and a requirement of premeiotic DNA replication. Based on these lines of evidence, we present the whole picture of molecular mechanism of VDEinitiated homing in yeast cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|