Effect of Particle Wettability and Particle Concentration on the Enzymatic Dehydration of n ‐Octanaloxime in Pickering Emulsions

Autor: Alessa Hinzmann, Ana Maria Bago Rodriguez, Harald Gröger, Lukas Schober, Bernard P. Binks
Rok vydání: 2021
Předmět:
Zdroj: Angewandte Chemie International Edition
Angewandte Chemie (International Ed. in English)
ISSN: 1433-7851
DOI: 10.1002/anie.202013171
Popis: Pickering emulsion systems have emerged as platforms for the synthesis of organic molecules in biphasic biocatalysis. Herein, the catalytic performance was evaluated for biotransformation using whole cells exemplified for the dehydration of n‐octanaloxime to n‐octanenitrile catalysed by an aldoxime dehydratase (OxdB) overexpressed in E. coli. This study was carried out in Pickering emulsions stabilised solely with silica particles of different hydrophobicity. We correlate, for the first time, the properties of the emulsions with the conversion of the reaction, thus gaining an insight into the impact of the particle wettability and particle concentration. When comparing two emulsions of different type with similar stability and droplet diameter, the oil‐in‐water (o/w) system displayed a higher conversion than the water‐in‐oil (w/o) system, despite the conversion in both cases being higher than that in a “classic” two‐phase system. Furthermore, an increase in particle concentration prior to emulsification resulted in an increase of the interfacial area and hence a higher conversion.
The dehydration of n‐octanaloxime to n‐octanenitrile catalysed by an aldoxime dehydratase (OxdB) overexpressed in E. coli is carried out in Pickering emulsions stabilised solely with silica particles of different hydrophobicity. The effects of particle wettability and particle concentration are evaluated with respect to the conversion of the reaction.
Databáze: OpenAIRE
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