Aspartate aminotransferase activity in crevicular fluid from dental implants
| Autor: | Mauro Di Stilio, Michele Paolantonio, Giacinto Di Placido, Vincenzo Tumini, Alessandro Contento, Giuseppe Spoto |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male medicine.medical_specialty Bleeding on probing Dentistry Gastroenterology Osseointegration Statistics Nonparametric Crevicular fluid Gingivitis Predictive Value of Tests Internal medicine medicine Mucositis Humans Aspartate Aminotransferases Dental Restoration Failure Periodontitis Aged Dental Implants Analysis of Variance Stomatitis business.industry Dental Implantation Endosseous Mouth Mucosa Gingival Crevicular Fluid Clinical Enzyme Tests Middle Aged medicine.disease Prognosis Cross-Sectional Studies Predictive value of tests Periodontics Female Analysis of variance medicine.symptom business Aminotransferase activity |
| Zdroj: | Journal of periodontology. 71(7) |
| ISSN: | 0022-3492 |
| Popis: | Aspartate aminotransferase (AST) is an enzyme normally confined to the cytoplasm of cells, but released to the extracellular environment upon cell death. Its levels are associated with the severity of experimental gingivitis and the loss of periodontal attachment. The aim of the present study was to investigate the presence and activity levels of AST in peri-implant crevicular fluid (PCF) from healthy and diseased endosseous implants in order to assess if AST in PCF can be further studied as a possible objective diagnostic aid in oral implantology.Eighty-one fixtures from 81 systemically healthy subjects were divided into 3 groups, 27 healthy implants (HI), 27 implants with mucositis (MI) and 27 implants affected by peri-implantitis (PI) according to well-defined clinical and radiographic criteria. PCF was collected by the insertion of a #40 standardized endodontic paper point to the base of the crevice or pocket for 30 seconds. AST activity was determined spectrophotometrically at 25 degrees C. The results were expressed as AST Units/ml in PCF.An AST activity was detected in each sample from HI, MI and PI. The mean AST activity in HI was 0.26 +/- 0.16 U/ml; in MI, 0.38 +/- 0.27 U/ml; in PI, 0.62 +/- 0.29 U/ml. ANOVA showed that the difference among HI, MI, and PI was statistically significant at P0.01 level. Post-hoc tests demonstrated that a significant difference in AST activity existed between HI/PI (t = 5.14; P0.01) and MI/PI (t = 3.09; P0.01). No statistically significant difference was found between HI/MI (t = 1.07; P0.1) AST activity was significantly (P0.01) associated with probing depth (r = 0.55), the amount of bone loss (r = 0.60) and bleeding on probing (r = 0.67). When the threshold for a positive AST test was setor =0.4 U/ml, a sensitivity = 0.81 and a specificity = 0.74 were found in the detection of peri-implantitis; the positive predictive value was 61% and the negative predictive value was 88%.Within the limits of this study, our results may suggest that PCF analysis could be further investigated in longitudinal studies as a suitable diagnostic strategy in the evaluation of dental implants. |
| Databáze: | OpenAIRE |
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