Cerebellar Cortical Demyelination in the Murine Cuprizone Model
| Autor: | Jens-Heiko Bussmann, Martin Stangel, Darius Moharregh-Khiabani, Maren Lindner, Refik Pul, Thomas Skripuletz, Viktoria Gudi, Paraskevi N. Koutsoudaki |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Cerebellum Time Factors Biology Corpus callosum Nerve Fibers Myelinated Pathology and Forensic Medicine White matter Cerebellar Cortex Cuprizone Mice Cortex (anatomy) medicine Animals Gliosis Remyelination Myelin Sheath Research Articles Cerebrum General Neuroscience Mice Inbred C57BL Disease Models Animal Oligodendroglia medicine.anatomical_structure nervous system Cerebral cortex Astrocytes Cerebellar cortex Microglia Neurology (clinical) Neuroscience Demyelinating Diseases |
| Zdroj: | Brain Pathol |
| ISSN: | 1750-3639 1015-6305 |
| DOI: | 10.1111/j.1750-3639.2009.00271.x |
| Popis: | In multiple sclerosis, demyelination occurs beside the white‐matter structures and in the cerebral and cerebellar cortex. We have previously shown that, in the cuprizone model, demyelination is present not only in the corpus callosum but also in the cerebral cortex. Here, we have performed a detailed analysis of the dynamics of de‐ and remyelination in the cerebellar cortex and white matter at nine timepoints in two cerebellar regions. To induce demyelination, C57BL/6 mice were fed with 0.2% cuprizone for 12 weeks followed by a recovery of 8 weeks. Both cortex and white‐matter structures were significantly demyelinated after 12 weeks of cuprizone feeding. Remyelination occurred after withdrawal of cuprizone but was less prominent in the more caudal cerebellar region. Microglia infiltration was prominent in all analyzed cerebellar areas, preceding demyelination by approximately 2–4 weeks, and was delayed in the more caudal cerebellar region. Astrogliosis was also seen but did not reach the extent observed in the cerebrum. In summary, cuprizone feeding provides an excellent model for the investigation of de‐ and remyelination processes in the cerebellar cortex and white matter. Furthermore, demyelination, microglia and astrocyte changes were different in the cerebellum as compared with the cerebrum, indicating region‐dependent pathomechanisms. |
| Databáze: | OpenAIRE |
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