Bivalent Peptide- and Chelator-Containing Bioconjugates as Toolbox Components for Personalized Nanomedicine
| Autor: | Lingzhou Zhao, Xiangyang Shi, Brigitte Voit, Albena Lederer, Andreas Janke, Dietmar Appelhans, Jennifer Daeg, Jinhua Zhao, Achim Temme, Xiaoying Xu, Susanne Boye |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Diagnostic Imaging
Glutamate Carboxypeptidase II Male Dendrimers Polymers and Plastics Biotin Contrast Media Bioengineering Peptide 02 engineering and technology 010402 general chemistry 01 natural sciences Polyethylene Glycols Biomaterials Heterocyclic Compounds 1-Ring chemistry.chemical_compound Folic Acid Materials Chemistry Animals Humans DOTA Molecular Targeted Therapy Epidermal growth factor receptor Chelating Agents chemistry.chemical_classification Mice Inbred BALB C biology Prostatic Neoplasms Avidin 021001 nanoscience & nanotechnology Xenograft Model Antitumor Assays Small molecule Combinatorial chemistry 0104 chemical sciences ErbB Receptors HEK293 Cells Nanomedicine chemistry Folate receptor Biotinylation Antigens Surface biology.protein Peptides 0210 nano-technology |
| Zdroj: | Biomacromolecules. 21:199-213 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.9b01127 |
| Popis: | While personalized therapy bears an enormous potential in cancer therapy, the development of flexible, tailorable delivery systems remains challenging. Here, we present a "tool-kit" of various avidin-based bioconjugates (BCs) for the preparation of personalized delivery systems. Corresponding BCs were synthesized using the self-assembly of avidin with various biotinylated ligands, such as one cationic glycodendrimer for dendriplex adsorption and two functional ligands for imaging (glycodendrimers with DOTA or NOTA units) or targeting (biotinylated PEG decorated with ligands). Substituting antibodies for targeting small molecules were coupled to biotin-PEG compounds for addressing the folate receptor (FR), epidermal growth factor receptor (EGFR), and prostate-specific membrane antigen (PSMA). After successful characterization and proof of good storage and redispersion properties of BCs, cytotoxicity assays and first in vivo imaging studies with 99mTc-complexing bioconjugates provide evidence that these BCs and their avidin analogues can be used as tool-kit components in theranostic systems for personalized medicine. |
| Databáze: | OpenAIRE |
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