Lipoprotein interactions with chromatic membranes as a novel marker for oxidative stress-related diseases
| Autor: | Nirit Hanin-Avraham, Angel Porgador, Sofiya Kolusheva, Michael Aviram, Agnieszka Mech-Dorosz, Bianca Fuhrman, Raz Jelinek |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Blood lipoprotein Lipid Bilayers Cell Biophysics medicine.disease_cause Biochemistry Fluorescence Membrane Lipids Diabetes mellitus Fluorescence Resonance Energy Transfer medicine Humans Membranes Chemistry Vesicle Cell Biology Middle Aged medicine.disease Lipoproteins LDL Oxidative Stress Membrane Förster resonance energy transfer medicine.anatomical_structure Diabetes Mellitus Type 2 Case-Control Studies lipids (amino acids peptides and proteins) Lipoproteins HDL Oxidation-Reduction Biomarkers Oxidative stress Lipoprotein |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1788(11):2436-2443 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/j.bbamem.2009.09.013 |
| Popis: | Changes in the abundance and properties of blood lipoproteins are generally considered major causes for varied pathological conditions and diseases. Using novel chromatic biomimetic vesicle and cell assays, we present here for the first time evidence for significant changes in lipoproteins' interactions with artificial membranes. Specifically, we demonstrate significant differences in membrane binding between lipoproteins (both low-density lipoprotein [LDL] and high-density lipoprotein [HDL]) harvested from diabetic patients vs. healthy controls as well as between oxidized and native lipoproteins. The chromatic assays, complemented by biophysical techniques and electron microscopy, point to significant reduction of surface membrane binding of the lipoproteins as a consequence of diabetes or oxidation. Overall, our results indicate that the substantial modulation of membrane interactions revealed by the chromatic assays may be used as a new and potentially powerful marker for screening and prediction of diseases associated with oxidative stress. |
| Databáze: | OpenAIRE |
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