A Simple Method for the Extraction and Purification of Human Growth Hormone and Its Assay by Paper Chromatography
| Autor: | J. M. C. Hoog, E. U. Bieler, S. W. Stroud |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Chromatography
Paper Endocrinology Diabetes and Metabolism Clinical Biochemistry Radioimmunoassay Alcohol Biochemistry Acetone chemistry.chemical_compound Endocrinology Nitration Methods Chemical Precipitation Humans chemistry.chemical_classification Chromatography Chemistry Biochemistry (medical) Extraction (chemistry) Polymer Electrophoresis Disc Paper chromatography Monomer Sephadex Growth Hormone Pituitary Gland Chromatography Gel Biological Assay |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 37:860-866 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-37-6-860 |
| Popis: | A simple method for the extraction and purification of human growth hormone has been developed, by which, after the initial alkaline extraction of the acetone-dried pituitary powder, the bulk of the impurities is precipitated at pH 4.8 and the crude hormone subsequently precipitated with alcohol at 25% v/v concentration. The crude hormone is then purified by a single Sephadex G-100 gel nitration, 75% of the activity of the crude being obtained as virtually pure monomer and the remaining 25% in the form of two fractions containing two separate polymers. A simple and rapid paper chromatographic assay method has also been developed for following the extraction and purification stages which gives good agreement with the corresponding radioimmunoassays. |
| Databáze: | OpenAIRE |
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