Prevalence of Epstein–Barr virus in tonsils and adenoids of United Arab Emirates nationals
| Autor: | Aktham Awwad, Ahmed Shams, Shamma Al Dhaheri, Suhail Al-Salam, Sayel Daoud, Mouied Al Ashari |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Epstein-Barr Virus Infections Herpesvirus 4 Human medicine.medical_treatment Tonsillitis medicine.disease_cause Severity of Illness Index Adenoidectomy Cohort Studies Immunophenotyping hemic and lymphatic diseases Prevalence Child In Situ Hybridization CD20 biology Biopsy Needle General Medicine Tonsils Immunohistochemistry UAE Child Preschool Female Antibody Adolescent United Arab Emirates Article Virus Young Adult Age Distribution EBV otorhinolaryngologic diseases medicine Humans Sex Distribution Epstein–Barr virus infection Retrospective Studies Tonsillectomy business.industry Infant medicine.disease Epstein–Barr virus Virology stomatognathic diseases Otorhinolaryngology Adenoids Pediatrics Perinatology and Child Health Immunology biology.protein business |
| Zdroj: | International Journal of Pediatric Otorhinolaryngology |
| ISSN: | 0165-5876 |
| DOI: | 10.1016/j.ijporl.2011.06.012 |
| Popis: | Objective Given that Epstein–Barr virus (EBV) often inhabits human tonsils and adenoids, it remains to be distinctively determined its prevalence and in which cell and microenvironment the virus is present. Methods To determine the prevalence of EBV in the tonsils and adenoids of the United Arab Emirates (UAE) nationals and to provide a basis for understanding the origin and biology of EBV-infected cells, the immunophenotype of all EBV-infected cells in 46 tonsils and 46 adenoids was determined by EBER in situ hybridization and immunohistochemistry with monoclonal antibodies to T cells (CD3), B cells (CD20), and epithelial cells (cytokeratin AE1/AE3), as well as immunostaining with antibodies to EBV latent membrane protein-1 (LMP-1). Results EBV was found in 43% of tonsillectomy specimens and 15% of adenoidectomy specimens. All EBV-infected cells were found to be B lymphocytes. About 90% of the infected B cells are found in the interfollicular regions of tonsils and adenoids and the remaining 10% are found within the follicles. There is no significant association between EBV infection, age ( P = 0.324) and gender ( P = 0.442). Conclusion EBV is associated with tonsillar hypertrophy and is prevalent in 43% of our cases. EBV is only detected in B lymphocytes and we believe that B lymphocytes are sites of primary infection and latency. In situ hybridization is the gold standard for the detection of EBV in tissue. |
| Databáze: | OpenAIRE |
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