Morphological changes in the cerebellum as a result of ethanol treatment and cigarette smoke exposure: A study on astrogliosis, apoptosis and Purkinje cells
| Autor: | Lucas Silva Tortorelli, Marília M. Wallauer, Rosalva Thereza Meurer, Fabiano B. Carvalho, Fernanda Huf, Francine Luciano Rahmeier, Marilda da Cruz Fernandes |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Cerebellum Apoptosis White matter Purkinje Cells 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Smoke Internal medicine mental disorders medicine Animals Gliosis Rats Wistar Cell Shape Ethanol Glial fibrillary acidic protein biology business.industry General Neuroscience medicine.disease Rats Astrogliosis 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Astrocytes biology.protein Immunohistochemistry Tobacco Smoke Pollution business 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Letters. 672:70-77 |
| ISSN: | 0304-3940 |
| Popis: | The link between Ethanol (EtOH) and tobacco (TOB) has potentially important implications for people involved in alcohol treatment; many alcoholics smoke, putting them at high risk of tobacco-related complications. The present study investigates the effect of chronic exposure to cigarette smoke, EtOH consumption and the combination of both on astrogliosis and apoptosis in the cerebellum of rats. Adult male Wistar rats were divided into 4 groups (8 animals per group): vehicle (glucose 3%, 10 mL/kg, twice a day), EtOH treated (EtOH 2 g/kg, twice a day), exposure to cigarette smoke (TOB, smoke of 6 cigarettes, twice a day) and a combination of EtOH and cigarette smoke (TOB + EtOH, twice a day). The treatment period was 57 days, after which the animals were euthanized, the cerebellum removed and subjected to immunohistochemical studies focusing on glial fibrillary acidic protein (GFAP), cleaved caspase-3, and S100. We also counted the number of Purkinje cells (PC) present following treatment. The combination of both EtOH and TOB exposure induced an increase in GFAP immunoreactivity, whilst TOB alone increased apoptosis in the white matter of the cerebellum. In addition, EtOH consumption reduced the number of PC and TOB tempered this effect. Overall, the present study opens up relevant perspectives for the consequences on human health of the combined use of alcohol and smoking, by demonstrating the biological mechanisms and cerebellar function vulnerabilities to combined use and dependence of licit drugs. |
| Databáze: | OpenAIRE |
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