Determination of Flavonoids in Licorice Using Acid Hydrolysis and Reversed-Phase HPLC and Evaluation of the Chemical Quality of Cultivated Licorice
| Autor: | Akinori Tabata, Makio Shibano, Hitoshi Watanabe, Masahiko Taniguchi, Kazuo Ozaki, Kimiye Baba |
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| Rok vydání: | 2009 |
| Předmět: |
Saponin
Pharmaceutical Science High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Chalcones Drug Discovery Glycyrrhiza Chromatography High Pressure Liquid Pharmacology chemistry.chemical_classification Detection limit Chromatography biology Chemistry Organic Chemistry Glycyrrhiza uralensis Glycoside Agriculture biology.organism_classification Isoflavones Complementary and alternative medicine Biochemistry Flavanones Molecular Medicine Liquiritigenin Isoliquiritigenin |
| Zdroj: | Planta Medica. 76:729-733 |
| ISSN: | 1439-0221 0032-0943 |
| Popis: | Licorice contains flavonoids and triterpenoids as the major bioactive components. Most of the flavonoids are the glycosidic form of liquiritigenin (LIQ), isoliquiritigenin (ISO) and formononetin (FOR). A reversed-phase HPLC method for the quantification of LIQ, ISO and FOR in licorice was developed. This method does not measure each glycoside but measures the aglycones using acid hydrolysis. All calibration curves showed good linear regression (r > 0.9998). The method showed good precision for intraday (RSD < 2.14 %) and interday (RSD < 0.51 %) assays. The limit of detection was 0.031 microg for LIQ, 0.011 microg for ISO and 0.006 microg for FOR. The limit of quantification was 0.31 microg for LIQ, 0.11 microg for ISO and 0.06 microg for FOR. The flavonoid contents along with the glycyrrhizin content of cultivated licorice from seedling plants in Japan and commercial wild licorice were investigated. This new method could be extremely useful for evaluating the quality of licorice. |
| Databáze: | OpenAIRE |
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