In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation
| Autor: | Paul DeSousa, Wim Bosma, J.R. Dobrinsky, Linda Harkness, Jie Zhu, H. David Guthrie, Ian Wilmut, Tim King, Lori L. Schreier, Tricia Ferrier |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Ovulation Swine Parthenogenesis Stimulation Fertilization in Vitro Biology Andrology chemistry.chemical_compound Embryonic and Fetal Development Human fertilization In vivo Pregnancy Culture Techniques medicine Animals Blastocyst Cytochalasin B reproductive and urinary physiology Fetus urogenital system Embryo Anatomy Embryo Transfer In vitro Electric Stimulation medicine.anatomical_structure chemistry embryonic structures Oocytes Female Developmental Biology Biotechnology |
| Zdroj: | Cloning and stem cells. 5(4) |
| ISSN: | 1536-2302 |
| Popis: | The objective of this study was to evaluate the in vitro and in vivo developmental competence of parthenogenetic (parthenote) pig embryos derived from ovulated and in vitro matured (IVM) oocytes. A total of four experiments were carried out. These demonstrated that the mean blastocyst rates from stimulated ovulated and IVM pig oocytes were not significantly different (61% vs. 46%, p > 0.05) following in vitro culture. Both ovulated and IVM pig parthenotes were able to develop in vivo for 30 days. Parthenote fetuses collected 21 and 30 days post estrus were morphologically normal but significantly smaller and lighter than fertilized controls (p < 0.01). IVM pig parthenotes stopped development around 31 days post estrus. |
| Databáze: | OpenAIRE |
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