NKG2D Polymorphism in Melanoma Patients from Southeastern Spain
| Autor: | Lourdes Gimeno, Helios Martínez-Banaclocha, Ruth López-Hernández, Luis Marín, Jose Miguel Bolarín, M.R. Moya-Quiles, Jorge A. Martínez-Escribano, Manuel Muro, José A. Campillo, M.R. Lopez-Alvarez, Maria Victoria Bernardo, José Francisco Frías-Iniesta, Alfredo Minguela, M.R Álvarez-López |
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| Přispěvatelé: | Facultad de Medicina, Departamento de Anatomía Humana |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Linkage disequilibrium gene polymorphism Single-nucleotide polymorphism chemical and pharmacologic phenomena Biology NKG2A lcsh:RC254-282 Article NKG2D 03 medical and health sciences 0302 clinical medicine SNP NK cell Melanoma Haplotype lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cutaneous melanoma Immunology Gene polymorphism CD8 |
| Zdroj: | DIGITUM. Depósito Digital Institucional de la Universidad de Murcia instname Cancers Cancers, Vol 11, Iss 4, p 438 (2019) Volume 11 Issue 4 DIGITUM: Depósito Digital Institucional de la Universidad de Murcia Universidad de Murcia |
| Popis: | Background: Natural killer (NK) and CD8+ T cells are involved in the immune response against melanoma. C-Type lectin-like NK cell receptors are located in the Natural Killer Complex (NKC) region 12p13.2-p12.3 and play a critical role in regulating the activity of NK and CD8+ T cells. An association between polymorphisms in the NKC region, including the NKG2D gene and NKG2A promoter, and the risk of cancer has been previously described. The aim of this study was to analyze the association of polymorphisms in the NKC region with cutaneous melanoma in patients from southeastern Spain. Methods: Seven single-nucleotide polymorphisms (SNPs) in the NKG2D gene (NKC3,4,7,9,10,11,12), and one SNP in the NKG2A promoter (NKC17) were genotyped by a TaqMan 5&prime Nuclease Assay in 233 melanoma patients and 200 matched healthy controls. Results: A linkage disequilibrium analysis of the SNPs performed in the NKC region revealed two blocks of haplotypes (Hb-1 and Hb-2) with 14 and seven different haplotype subtypes, respectively. The third most frequent haplotype from the block Hb-2&mdash NK3 (CAT haplotype)&mdash was significantly more frequent on melanoma patients than on healthy controls (p = 0.00009, Pc = 0.0006). No further associations were found when NKC SNPs were considered independently. Conclusions: Our results suggest an association between NKG2D polymorphisms and the risk of cutaneous malignant melanoma. |
| Databáze: | OpenAIRE |
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