Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill
| Autor: | João Carlos Bespalhok Filho, Marília Pereira Machado, Flávio Zanette, André Luís Lopes da Silva, Cícero Deschamps, Luiz Antonio Biasi |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Brazilian Archives of Biology and Technology, Volume: 57, Issue: 5, Pages: 636-643, Published: OCT 2014 Brazilian Archives of Biology and Technology, Vol 57, Iss 5, Pp 636-643 (2014) Brazilian Archives of Biology and Technology v.57 n.5 2014 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
| ISSN: | 1516-8913 |
| DOI: | 10.1590/s1516-8913201402165 |
| Popis: | In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities. |
| Databáze: | OpenAIRE |
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