Inhibition of Oxidation of Low-Density Lipoprotein by a Novel Antioxidant, BO-653, Prepared by Theoretical Design
| Autor: | Etsuo Niki, Yuko Okimoto, Jyunichi Tsuchiya, Noriko Noguchi, Tatsuhiko Kodama, Osamu Cynshi |
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| Rok vydání: | 1997 |
| Předmět: |
Lipid Peroxides
Antioxidant Arteriosclerosis medicine.medical_treatment Radical Amidines Biophysics Ascorbic Acid Oxidative phosphorylation Biochemistry Antioxidants Cyclic N-Oxides chemistry.chemical_compound Nitriles medicine Animals Humans Vitamin E Organic chemistry Molecular Biology Scavenging Apolipoproteins B Benzofurans Aqueous two-phase system Free Radical Scavengers Oxidants Ascorbic acid Peroxides Lipoproteins LDL Kinetics chemistry Drug Design Low-density lipoprotein lipids (amino acids peptides and proteins) Lipid Peroxidation Rabbits Azo Compounds Oxidation-Reduction Lipoprotein |
| Zdroj: | Archives of Biochemistry and Biophysics. 347:141-147 |
| ISSN: | 0003-9861 |
| DOI: | 10.1006/abbi.1997.0331 |
| Popis: | 2,3-Dihydro-5-hydroxy-2,2-dipentyl-4,6-di-tert-butyl-benzofuran (BO-653) is a novel antioxidant synthesized by theoretical designing based on the previous experimental findings and consideration. The antioxidant activities of BO-653 against the oxidative modification of low-density lipoprotein (LDL) induced by free radicals were studied. BO-653 was consumed faster than endogenous alpha-tocopherol and inhibited the formation of lipid hydroperoxides, which was observed during the consumption of alpha-tocopherol. Doxyl stearic acids incorporated into LDL as spin probes competed with the antioxidants in scavenging radicals. It was found that the efficacy of radical scavenging by alpha-tocopherol became smaller as the radical went deeper into the interior of LDL particle, whereas that by BO-653 did not change. Ascorbic acid in the aqueous phase spared alpha-tocopherol efficiently during oxidation. On the other hand, the sparing effect of ascorbic acid for BO-653 was not remarkable, unlike that for alpha-tocopherol, which implied different locations of radicals derived from BO-653 and alpha-tocopherol within the LDL particle. It was concluded that BO-653 protected LDL from oxidative modification efficiently by scavenging peroxyl radicals and by reducing alpha-tocopheroxyl radicals and that this novel antioxidant might act as a potent inhibitor of development of atherosclerosis. |
| Databáze: | OpenAIRE |
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