Genomic, Proteomic and Morphological Characterization of Two Novel Broad Host Lytic Bacteriophages ΦPD10.3 and ΦPD23.1 Infecting Pectinolytic Pectobacterium spp. and Dickeya spp
| Autor: | Victor de Jager, Adam Ossowicki, Anna Smolarska, Joanna Siwinska, Robert Czajkowski, Ewa Lojkowska, Zofia Ozymko, Magdalena Narajczyk |
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| Přispěvatelé: | Microbial Ecology (ME) |
| Rok vydání: | 2015 |
| Předmět: |
Proteomics
Pectobacterium Science Pectobacterium carotovorum Myoviridae Dickeya Host Specificity Microbiology 03 medical and health sciences Enterobacteriaceae Caudovirales Pulsed-field gel electrophoresis Bacteriophages Plant Diseases Solanum tuberosum 030304 developmental biology 0303 health sciences Multidisciplinary biology 030306 microbiology food and beverages biology.organism_classification Plant Tubers Lytic cycle international Medicine Adsorption Dickeya solani Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 3, p e0119812 (2015) PLoS One, 10(3):e0119812. Public Library of Science |
| ISSN: | 1932-6203 |
| Popis: | Pectinolytic Pectobacterium spp. and Dickeya spp. are necrotrophic bacterial pathogens of many important crops, including potato, worldwide. This study reports on the isolation and characterization of broad host lytic bacteriophages able to infect the dominant Pectobacterium spp. and Dickeya spp. affecting potato in Europe viz. Pectobacterium carotovorum subsp. carotovorum (Pcc), P. wasabiae (Pwa) and Dickeya solani (Dso) with the objective to assess their potential as biological disease control agents. Two lytic bacteriophages infecting stains of Pcc, Pwa and Dso were isolated from potato samples collected from two potato fields in central Poland. The ΦPD10.3 and ΦPD23.1 phages have morphology similar to other members of the Myoviridae family and the Caudovirales order, with a head diameter of 85 and 86 nm and length of tails of 117 and 121 nm, respectively. They were characterized for optimal multiplicity of infection, the rate of adsorption to the Pcc, Pwa and Dso cells, the latent period and the burst size. The phages were genotypically characterized with RAPD-PCR and RFLP techniques. The structural proteomes of both phages were obtained by fractionation of phage proteins by SDS-PAGE. Phage protein identification was performed by liquid chromatography-mass spectrometry (LC-MS) analysis. Pulsed-field gel electrophoresis (PFGE), genome sequencing and comparative genome analysis were used to gain knowledge of the length, organization and function of the ΦPD10.3 and ΦPD23.1 genomes. The potential use of ΦPD10.3 and ΦPD23.1 phages for the biocontrol of Pectobacterium spp. and Dickeya spp. infections in potato is discussed. |
| Databáze: | OpenAIRE |
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