Vitellogenin as biomarker for estrogenicity in flounder Platichthys flesus in the field and exposed to 17α-ethinylestradiol via food and water in the laboratory
| Autor: | Poul Bjerregaard, Bodil Korsgaard, Thomas Aagaard, Louise Leonharder Madsen, Knud Ladegaard Pedersen, Lisette Bachmann Bjerregaard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Ethinyl Estradiol/administration & dosage Wet weight Denmark Administration Oral Flounder Oceanography Ethinyl Estradiol Vitellogenins Liver/drug effects Testis Tissue Distribution Flounder/blood Water Pollutants Chemical/analysis biology General Medicine Pollution Platichthys 17α ethinylestradiol Liver Fish medicine.medical_specialty Dependent manner Platichthys flesus 17α-Ethinylestradiol Vitellogenin Aquatic Science Body weight Dietary exposure Animal science Accumulation Estrogenic effect Internal medicine medicine Animals Body Weight/drug effects Dose-Response Relationship Drug Body Weight Environmental Exposure biology.organism_classification Endocrinology biology.protein Testis/drug effects Vitellogenins/blood Biomarkers Water Pollutants Chemical Biomarkers/blood Environmental Exposure/adverse effects |
| Zdroj: | Madsen, L L, Korsgaard, B, Pedersen, K L, Bjerregaard, L B, Aagaard, T & Bjerregaard, P 2013, ' Vitellogenin as biomarker for estrogenicity in flounder Platichthys flesus in the field and exposed to 17α-ethinylestradiol via food and water in the laboratory ', Marine Environmental Research, vol. 92, no. December, pp. 79-86 . https://doi.org/10.1016/j.marenvres.2013.09.002 |
| DOI: | 10.1016/j.marenvres.2013.09.002 |
| Popis: | The ability of 17α-ethinylestradiol (EE2) to elevate vitellogenin levels were investigated in male flounder Platichthys flesus and vitellogenin concentrations in flounders from the Danish coastal environment were determined. Male flounders were exposed to 17α-ethinylestradiol (EE2) via food or water. Average vitellogenin concentrations in the control fish ranged between 25 and 100ngmL -1. Exposure to 5.1, 8.1 and 16.8ng EE2 L -1 in water and 500 and 5000ng EE2 kg -1 body weight (bw) every second day in the food increased the plasma vitellogenin concentration in a concentration and time dependent manner, whereas exposure to 2.7ng EE2 L -1 in water for 21d and 5 and 50ng EE2 kg -1 bw for 12 days in the food did not. EE2 could be detected in liver and testes (but not in muscle) after exposure to 8.1 and 16.8ng EE2 L -1 in the water and 5000ng EE2 kg -1 bw in the food; the highest concentration was 6ng g -1 wet weight in liver. The majority of the male flounders collected from nine coastal Danish sites from 1999 to 2004 had vitellogenin concentrations below 100ngmL -1, and only at two sites moderate estrogenic inputs were indicated. |
| Databáze: | OpenAIRE |
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