Surface characterisation and biomechanical analysis of the sclera by atomic force microscopy
| Autor: | M. D. Savage, Colin A. Grant, Denis Greig, Neil H. Thomson, Hong W. Woon |
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| Rok vydání: | 2011 |
| Předmět: |
Materials science
genetic structures Surface Properties Swine Biomedical Engineering Connective tissue Microscopy Atomic Force Biomaterials Stroma medicine Animals Elasticity (economics) Elastic modulus Mechanical Phenomena biology Atomic force microscopy Anatomy Nanoindentation eye diseases Biomechanical Phenomena Sclera medicine.anatomical_structure Proteoglycan Mechanics of Materials biology.protein Biophysics sense organs |
| Zdroj: | Journal of the Mechanical Behavior of Biomedical Materials. 4:535-540 |
| ISSN: | 1751-6161 |
| DOI: | 10.1016/j.jmbbm.2010.12.011 |
| Popis: | The sclera is an important collagenous based connective tissue that gives the eye its shape and protects the sensitive layers within the globe. The elasticity and resilience of the sclera mainly come from the stroma, which contains a dense network of collagen fibrils comprising 90% of the thickness of the tissue. However, the outermost layer of the scleral tissue (∼10 μm) is known as the episclera, which is mostly uncharacterised and seldom investigated. Here, we use AFM scanning of porcine eyes to show that the surfaces of these two distinctive layers are structurally different. Furthermore, we use AFM nanoindentation to show that the episclera has significantly different mechanical properties than the stroma. The mechanical properties of the stroma are shown to be distributed between its two component parts (proteoglycan matrix and collagen fibrils). |
| Databáze: | OpenAIRE |
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