Membrane-active agents and heat-induced erythrocyte fragmentation
| Autor: | Harold S. Zarkowsky |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Diamide
Heat induced Erythrocytes Hot Temperature Dose-Response Relationship Drug Chemistry Chlorpromazine Erythrocyte Membrane p-Chloromercuribenzoic Acid Lysophosphatidylcholines Hematology In Vitro Techniques Dose–response relationship chemistry.chemical_compound Membrane Adenosine Triphosphate Ethylmaleimide Biophysics Humans Fragmentation (cell biology) P-Chloromercuribenzoic Acid Maleimide Chloromercuribenzoates |
| Zdroj: | British Journal of Haematology. 50:361-365 |
| ISSN: | 0007-1048 |
| DOI: | 10.1111/j.1365-2141.1982.00345.x-i1 |
| Popis: | Morphological observations of heated erythrocytes were made after preincubation with lysolecithin, diamide, p-chloromercuribenzoate, or N-ethyl maleimide and after ATP-depletion. Diamide (1.0 mM) reduced the critical temperature of fragmentation from 49 degrees C to 47 degrees C, and at higher concentrations the critical temperature was further reduced. The other sulphydryl reacting agents had little or no effect. Drug-induced spherocytes and ATP-depleted cells did not fragment. Membrane-active agents can effect the response of membranes to heat and the effect is dose-dependent. |
| Databáze: | OpenAIRE |
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