Physical association between a novel plasma-membrane structure and centrosome orients cell division
| Autor: | Kazuyoshi Murata, Naoyuki Miyazaki, Takefumi Negishi, Hitoyoshi Yasuo, Naoto Ueno |
|---|---|
| Přispěvatelé: | National Institutes of Natural Sciences, Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Observatoire océanologique de Villefranche-sur-mer (OOVM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), The Graduate University for Advanced Studies |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Centriole Cell division QH301-705.5 Science Centrosome cycle [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences spindle orientation Basal body Biology (General) Mitosis General Immunology and Microbiology General Neuroscience Cilium General Medicine Cell biology 030104 developmental biology Centrosome Ciliary positioning Medicine Interphase |
| Zdroj: | eLife eLife, eLife Sciences Publication, 2016, 5, pp.e16550. ⟨10.7554/eLife.16550⟩ eLife, 2016, 5, pp.e16550. ⟨10.7554/eLife.16550⟩ eLife, Vol 5 (2016) |
| ISSN: | 2050-084X |
| Popis: | International audience; In the last mitotic division of the epidermal lineage in the ascidian embryo, the cells divide stereotypically along the anterior-posterior axis. During interphase, we found that a unique membrane structure invaginates from the posterior to the centre of the cell, in a microtubule-dependent manner. The invagination projects toward centrioles on the apical side of the nucleus and associates with one of them. Further, a cilium forms on the posterior side of the cell and its basal body remains associated with the invagination. A laser ablation experiment suggests that the invagination is under tensile force and promotes the posterior positioning of the centrosome. Finally, we showed that the orientation of the invaginations is coupled with the polarized dynamics of centrosome movements and the orientation of cell division. Based on these findings, we propose a model whereby this novel membrane structure orchestrates centrosome positioning and thus the orientation of cell division axis. |
| Databáze: | OpenAIRE |
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