Detection of recombinant bovine somatotropin in milk and effect of industrial processes on its stability
| Autor: | Gaud Pinel, Sandrine Rochereau-Roulet, Janique Richoz-Payot, Marie-Hélène Le Breton, Andrea Beck-Henzelin, Bruno Le Bizec, Thierry Delatour |
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| Rok vydání: | 2010 |
| Předmět: |
Reproducibility
Chromatography Chemistry Protein Stability Pasteurization Repeatability Biochemistry Defatting Mass Spectrometry Analytical Chemistry law.invention Milk law Growth Hormone Environmental Chemistry media_common.cataloged_instance Animals Sample preparation Bovine somatotropin Cattle Solid phase extraction European union Spectroscopy Chromatography High Pressure Liquid media_common |
| Zdroj: | Analytica chimica acta. 672(1-2) |
| ISSN: | 1873-4324 |
| Popis: | A LC-MS/MS method has been developed for the direct detection of recombinant bovine somatotropin (rbST) in milk and dairy products. The sample preparation protocol is based on a solid phase extraction step followed by precipitation with cold methanol and enzymatic digestion. The analysis is focused on the tryptic N-terminal peptide, specific of the recombinant form of the hormone and the detection is performed by LC-ESI(+)-MS/MS. This method has been validated according to the European Union criteria described in the Directive 2002/657/EC. Acceptable performances, with a decision limit (CCalpha) of 1.24 ng mL(-1) and detection capability (CCbeta) of 1.92 ng mL(-1) were obtained. Calculation of repeatability and intermediate reproducibility of the signal at 100 ng mL(-1) lead to relative standard deviations lower than 20%, showing the robustness of the method. Samples subjected to various industrial processes namely, heating, freezing, defatting, pasteurization and spray-drying were then analysed in order to determine the consequences of these treatments on the stability of the hormone. Results showed that temperature related processes, such as pasteurization and spray-drying induce a loss of the hormone up to 95%. |
| Databáze: | OpenAIRE |
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