Evaluation of commonly used tear sampling methods and their relevance in subsequent biochemical analysis
| Autor: | Aniko Rentka, Krisztina Köröskényi, Peter Szodoray, Jolan Harsfalvi, Gabriella Szücs, Zoltán Szekanecz, Adam Kemeny-Beke |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Bioactive molecules Clinical Biochemistry Bioinformatics 03 medical and health sciences 0302 clinical medicine Diagnosis Humans Medicine Relevance (information retrieval) Elméleti orvostudományok Body fluid Clinical Laboratory Techniques business.industry Relative distribution Orvostudományok General Medicine eye diseases 030104 developmental biology Tears 030221 ophthalmology & optometry sense organs business |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 54:521-529 |
| ISSN: | 1758-1001 0004-5632 |
| Popis: | The human precorneal tear film is a special body fluid, since it is a complex mixture of proteins, lipids, small bioactive molecules, and their concentrations and relative distribution represent not only the metabolic state of the ocular surface but also the systemic and local homeostasis of the outer eye and the human body. This suggests that biochemical analysis of the precorneal tear film composition may provide a non-invasive tool for diagnosis and monitoring of disease progression or treatment efficacy in human medicine. However, collecting tears is demanding, and obtaining reproducible and unaltered samples is challenging because of the small sample volumes of tears. Several methods are available for tear collection as a preparatory step of precorneal tear film analysis, and the collection method used has to be assessed since it has a critical impact on the effectiveness of the assays and on the quality of the results. Each sampling method has advantages and disadvantages; therefore, it is not easy to choose the appropriate collecting method for tear collection. To overcome these limitations various methods have been recommended by different authors for special aspects of specific tests. The aim of our review was to evaluate tear sampling methods with regard to our ongoing biochemical analysis. *Contributed equally. |
| Databáze: | OpenAIRE |
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