Nitric oxide synthase reduces nitrite to NO under anoxia
| Autor: | Anatoly F. Vanin, Anny Slama-Schwok, L. M. Bevers, E. E. van Faassen |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Arginine Nitric Oxide Synthase Type III medicine.drug_class Cell Survival Endothelial NOS Nitric Oxide Nitric oxide Cell Line Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Enos Internal medicine medicine Animals Nitrite Molecular Biology Xanthine oxidase inhibitor Nitrites Pharmacology biology Endothelial Cells Immunotherapy gene therapy and transplantation [UMCN 1.4] Cell Biology Nitrite reductase biology.organism_classification Cell Hypoxia Nitric oxide synthase Kinetics Endocrinology chemistry Biochemistry biology.protein Molecular Medicine Oxidation-Reduction Immunity infection and tissue repair [NCMLS 1] |
| Zdroj: | Cellular and Molecular Life Sciences, 64, 1, pp. 96-103 Cellular and Molecular Life Sciences, 64, 96-103 |
| ISSN: | 1420-682X |
| Popis: | Item does not contain fulltext Cultured bEND.3 endothelial cells show a marked increase in NO production when subjected to anoxia, even though the normal arginine pathway of NO formation is blocked due to absence of oxygen. The rate of anoxic NO production exceeds basal unstimulated NO synthesis in normoxic cells. The anoxic release of NO is mediated by endothelial nitric oxide synthase (eNOS), can be abolished by inhibitors of NOS and is accompanied by consumption of intracellular nitrite. The anoxic NO release is unaffected by the xanthine oxidase inhibitor oxypurinol. The phenomenon is attributed to anoxic reduction of intracellular nitrite by eNOS, and its magnitude and duration suggests that the nitrite reductase activity of eNOS is relevant for fast NO delivery in hypoxic vascular tissues. |
| Databáze: | OpenAIRE |
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