Amelioration of regulatory T cells by Lactobacillus delbrueckii and Lactobacillus rhamnosus in pristane-induced lupus mice model

Autor: Fahimeh Lavi Arab, Maryam Rastin, Seyed-Alireza Esmaeili, Sahar Khorasani, Mahmoud Mahmoudi, Mahmoud Reza Kalantari, Nafiseh Tabasi, Fatemeh Mardani
Rok vydání: 2018
Předmět:
0301 basic medicine
Anti-nuclear antibody
Physiology
Regulatory T cell
Clinical Biochemistry
Anti-Inflammatory Agents
Kidney Function Tests
T-Lymphocytes
Regulatory
Antibodies
03 medical and health sciences
0302 clinical medicine
Immune system
Lactobacillus rhamnosus
immune system diseases
medicine
Animals
Lupus Erythematosus
Systemic
Urea
skin and connective tissue diseases
Interleukin 6
Cell Proliferation
Lactobacillus delbrueckii
Mice
Inbred BALB C
Systemic lupus erythematosus
Granuloma
biology
business.industry
Lacticaseibacillus rhamnosus
Terpenes
Probiotics
food and beverages
Peripheral tolerance
FOXP3
Forkhead Transcription Factors
Cell Biology
medicine.disease
biology.organism_classification
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Creatinine
Immunology
biology.protein
Cytokines
Female
business
Zdroj: Journal of cellular physiology. 234(6)
ISSN: 1097-4652
Popis: Regulatory T cells (Tregs) play an indispensable role in the control of immune responses and induction of peripheral tolerance. Dysregulation of Tregs is involved in the pathogenesis of systemic lupus erythematosus (SLE). Tolerogenic probiotics have shown beneficial effects in the control of autoimmune diseases. We evaluated the prophylactic and therapeutic effects of Lactobacillus delbrueckii and Lactobacillus rhamnosus on Tregs and their related molecules in pristane-induced lupus mice model. Fifty-four female BALB/c mice (3-5 weeks) were randomly divided into nine groups. Lupus was induced in all groups using pristane. Prophylactic groups were treated from Day 0 (at the time of pristane injection) and treatment groups were treated 2 months later with L. rhamnosus, L. delbrueckii, mix of both probiotics, and prednisolone. One group was considered as SLE-induced control group without any treatment. Presence of antinuclear antibodies (ANA), antidouble-stranded DNA (anti-dsDNA), antiribonucleoprotein (anti-RNP), proteinuria, and serum level of creatinine, urea, the expression of forkhead box P3 (Foxp3), interleukin 6 (IL-6), IL-10, transforming growth factor β, and the number of Tregs were determined. SLE induction by pristane led to the formation of lipogranuloma, presence of ANA, anti-dsDNA, and anti-RNP. Probiotics consumption decreased the level of lipogranuloma, ANA, and anti-dsDNA. In addition, in probiotics receiving groups, Tregs and the expression level of Foxp3 increased, while IL-6 decreased. The effect of probiotics in the prophylactic group was more prominent. The results may indicate the effectiveness of L. delbrueckii and L. rhamnosus in the enhancement of Tregs and the decrease of inflammatory cytokines and disease severity in SLE-induced mice.
Databáze: OpenAIRE
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