Enhanced tolerance of transgenic potato plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against oxidative stress and high temperature
| Autor: | Jin-Seog Kim, Sun-Hyung Kim, Kwang Yun Cho, Suk-Yoon Kwon, Jung Sup Choi, Li Tang, Sang-Soo Kwak, Chang K. Sung, Haeng-Soon Lee |
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| Rok vydání: | 2006 |
| Předmět: |
Paraquat
Chloroplasts Antioxidant medicine.medical_treatment Plant Science Oxidative phosphorylation Photosynthesis medicine.disease_cause Superoxide dismutase Ascorbate Peroxidases Gene Expression Regulation Plant medicine Solanum tuberosum biology Reverse Transcriptase Polymerase Chain Reaction Superoxide Dismutase fungi Temperature food and beverages General Medicine Plants Genetically Modified biology.organism_classification Adaptation Physiological Plant Leaves Chloroplast Oxidative Stress stomatognathic diseases Peroxidases Biochemistry biology.protein Agronomy and Crop Science Solanaceae Oxidative stress Peroxidase |
| Zdroj: | Plant Cell Reports. 25:1380-1386 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-006-0199-1 |
| Popis: | Oxidative stress is a major damaging factor for plants exposed to environmental stresses. In order to develop transgenic potato plants with enhanced tolerance to environmental stress, the genes of both Cu/Zn superoxide dismutase and ascorbate peroxidase were expressed in chloroplasts under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SSA plants). SSA plants showed enhanced tolerance to 250 microM methyl viologen, and visible damage in SSA plants was one-fourth that of non-transgenic (NT) plants that were almost destroyed. In addition, when SSA plants were treated with a high temperature of 42 degrees C for 20 h, the photosynthetic activity of SSA plants decreased by only 6%, whereas that of NT plants decreased by 29%. These results suggest that the manipulation of the antioxidative mechanism of the chloroplasts may be applied in the development of industrial transgenic crop plants with increased tolerance to multiple environmental stresses. |
| Databáze: | OpenAIRE |
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