Mapping of spinal interneurons involved in regulation of the lower urinary tract in juvenile male rats
| Autor: | Sergei Karnup, W.C. de Groat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Lf lateral funiculus SPPN spinal parasympathetic preganglionic neuron W_R weak/moderate red BL bladder B_G bright green CC - central canal Synapse RFP red fluorescent protein 0302 clinical medicine BCM bulbocavernosus muscle Glycine receptor GFP green fluorescent protein education.field_of_study Spinal cord IML intermediolateral nucleus General Neuroscience IN interneuron Commissure B_R bright red Cell biology medicine.anatomical_structure GABAergic PRV pseudorabies virus Population SC spinal cord Biology Inhibitory postsynaptic potential DSD detrusor-sphincter-dyssynergia Article lcsh:RC321-571 Pseudorabies virus 03 medical and health sciences SCI spinal cord injury VMf ventro-medial funiculus medicine education lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry DCM dorsal commissure EUS external urethral sphincter Transsynaptic tracing PPN propriospinal neuron fungi Colocalization sIN secondary interneuron digestive system diseases EMG electromyogram 030104 developmental biology EUS-MN motoneuron of the external urethral sphincter nervous system pIN primary interneuron LUT lower urinary tract W_G weak/moderate green LSCC lumbar spinal coordinating center 030217 neurology & neurosurgery |
| Zdroj: | IBRO Reports IBRO Reports, Vol 9, Iss, Pp 115-131 (2020) |
| ISSN: | 2451-8301 |
| Popis: | Coordination between the urinary bladder (BL) and external urethral sphincter (EUS) is necessary for storage and elimination of urine. In rats interneuronal circuits at two levels of the spinal cord (i.e., L6-S1 and L3-L4) play an important role in this coordination. In the present experiments retrograde trans-synaptic transport of pseudorabies virus (PRV) encoding fluorescent markers (GFP and RFP) was used to trace these circuits. To examine the relative localization of EUS-related and BL-related interneuronal populations we injected PRV-GFP into the EUS and PRV-RFP into the BL wall. The PRV infected populations of spinal interneurons were localized primarily in the dorsal commissure (DCM) of L6/S1 and in a hypothesized lumbar spinal coordinating center (LSCC) in L3/L4 above and lateral to central canal (CC). At both sites colocalization of markers occurred in a substantial number of labeled interneurons indicating concomitant involvement of these double-labelled neurons in the EUS- and BL-circuits and suggesting their role in EUS-BL coordination. Intense GFP or RFP fluorescent was detected in a subpopulation of cells at both sites suggesting that they were infected earlier and therefore likely to represent first order, primary interneurons that directly synapse with output neurons. Larger numbers of weakly fluorescent neurons that likely represent second order interneurons were also identified. Within the population of EUS-related first order interneurons only 3-8 % exhibited positive immunoreaction for an early transcription factor Pax2 specific to GABAergic and glycinergic inhibitory neurons suggesting that the majority of interneurons in DCM and LSCC projecting directly to the EUS motoneurons are excitatory. |
| Databáze: | OpenAIRE |
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