Metagenomic Analysis of Gingival Sulcus Microbiota and Pathogenesis of Periodontitis Associated with Type 2 Diabetes Mellitus
| Autor: | A. G. Gabibov, V. N. Tsarev, S. N. Kostryukova, E. S. Vakhitova, S D Arutyunov, I. P. Balmasova, Elena N. Ilina, E. A. Babaev, Ashot Musaelovich Mkrtumyan |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Dental Plaque Gingiva 030209 endocrinology & metabolism Dental plaque Aggregatibacter actinomycetemcomitans General Biochemistry Genetics and Molecular Biology Pathogenesis 03 medical and health sciences 0302 clinical medicine RNA Ribosomal 16S Diabetes mellitus medicine Humans Gingival sulcus Periodontitis business.industry Biofilm Type 2 Diabetes Mellitus General Medicine Middle Aged medicine.disease stomatognathic diseases 030104 developmental biology Diabetes Mellitus Type 2 Biofilms Case-Control Studies Chronic Periodontitis Immunology Metagenome Female business Porphyromonas gingivalis Treponema denticola Gingival disease |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 163:718-721 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-017-3888-6 |
| Popis: | Biofilm of the gingival sulcus from 22 patients with type 2 diabetes mellitus and periodontitis, 30 patients with periodontitis not complicated by diabetes mellitus (reference group), and 22 healthy volunteers without signs of gingival disease (control group) was studied by quantitative PCR. Quantitative analysis for the content of P. gingivalis, T. forsythia, A. ctinomycetemcomitans, T. denticola, P. intermedia, F. nucleatum/periodonticum, and P. endodontalis in the dental plaque was performed with a Dentoscreen kit. The presence of other bacterial groups was verified by metagenomic sequencing of the 16S rRNA gene to evaluate some specific features of the etiological factor for periodontitis in type 2 diabetes mellitus. Specimens of the Porphiromonadaceae and Fusobacteriaceae families were characterized by an extremely high incidence in combined pathology. The amount of Sphingobacteriaceae bacteria in the biofilm was shown to decrease significantly during periodontitis. Metagenomic analysis confirmed the pathogenic role of microbiota in combined pathology, as well as the hypothesis on a possible influence of periodontitis on the course and development of type 2 diabetes mellitus. |
| Databáze: | OpenAIRE |
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