Proteomic effects of repeated-dose oral exposure to 2-monochloropropanediol and its dipalmitate in rat testes
| Autor: | Axel Oberemm, Albert Braeuning, Falko Frenzel, Alfonso Lampen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Glycerol
Male Proteomics 0301 basic medicine Testicular tissue MAP Kinase Signaling System Palmitic Acid Administration Oral Food Contamination Pharmacology Toxicology 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Isomerism Oral administration Testis 3-MCPD Animals Rats Wistar chemistry.chemical_classification Dose-Response Relationship Drug Chemistry Proteins Fatty acid Fatty acid ester 04 agricultural and veterinary sciences General Medicine 040401 food science Mitochondria 030104 developmental biology Infant formula 2-monochloropropanediol Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Toxicity Energy Metabolism Food Science |
| Zdroj: | Food and Chemical Toxicology. 116:354-359 |
| ISSN: | 0278-6915 |
| Popis: | 2- and 3-monochloropropanediol (2-MCPD) and their fatty acid esters are food contaminants which are concomitantly formed upon thermal treatment of foodstuff containing fats and salt. Exposure to 2- or 3-MCPD thus results, for example, from refined vegetable oils, in instant meals or infant formula, as well as in cereals or pastries. The molecular mechanisms of 2-MCPD toxicity are poorly understood. Here, we performed a comprehensive proteomic analysis of 2-MCDP-induced alterations in the testes from rats following oral administration of 10 mg/kg body weight per day 2-MCPD, or an equimolar dose of 2-MCPD dipalmitate as a representative 2-MCPD fatty acid ester. In the absence of overt histopathologically detectable toxicity, moderate alterations in cellular proteomic signatures were recorded. The observations are in line with the assumption that the molecular mechanisms of 2-MCPD and 3-MCPD toxicity differ. Observed proteomic alterations point towards effects of 2-MCPD on mitogen-dependent signaling and mitochondrial energy utilization. Presented data for the first time provide insight into proteomic effects of 2-MCPD in testicular tissue. |
| Databáze: | OpenAIRE |
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